Penetratin-induced Aggregation and Subsequent Dissociation of Negatively Charged Phospholipid Vesicles
Journal article, 2001

The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from alpha -helix to antiparallel beta -pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed.

penetratin

Antennapedia homeodomain

vesicle aggregation

turbidity

artifact

circular dichroism

Author

Daniel Persson

Department of Physical Chemistry

Per Thoren

Department of Physical Chemistry

Bengt Nordén

Department of Physical Chemistry

FEBS Letters

0014-5793 (ISSN)

Vol. 505 2 307-312

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Energy

Life Science Engineering (2010-2018)

Materials Science

Subject Categories

Physical Chemistry

Roots

Basic sciences

DOI

10.1016/S0014-5793(01)02843-5

More information

Created

10/7/2017