Penetratin-induced Aggregation and Subsequent Dissociation of Negatively Charged Phospholipid Vesicles
Artikel i vetenskaplig tidskrift, 2001

The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from alpha -helix to antiparallel beta -pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed.

penetratin

Antennapedia homeodomain

vesicle aggregation

turbidity

artifact

circular dichroism

Författare

Daniel Persson

Institutionen för fysikalisk kemi

Per Thoren

Institutionen för fysikalisk kemi

Bengt Nordén

Institutionen för fysikalisk kemi

FEBS Letters

0014-5793 (ISSN)

Vol. 505 2 307-312

Styrkeområden

Nanovetenskap och nanoteknik

Energi

Livsvetenskaper och teknik

Materialvetenskap

Ämneskategorier

Fysikalisk kemi

Fundament

Grundläggande vetenskaper

DOI

10.1016/S0014-5793(01)02843-5