Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery
Journal article, 2011

Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic equilibrium. Moreover, dendrimer surface modification through PEGylation appears to make the interaction with DNA more reversible, which is favorable from the perspective of vector unpacking. Probing the nonequilibrium state of DNA during condensation processes is thus important for developing novel vectors, and further, it could also be useful in the study of chromatin folding.

starburst dendrimers

complexes

acetylation

pamam dendrimers

oligonucleotides

polyamidoamine dendrimers

efficiency

cytotoxicity

transfection

bromide

antisense

Author

Kristina Fant

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Bengt Nordén

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Per Lincoln

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Biochemistry

0006-2960 (ISSN) 1520-4995 (eISSN)

Vol. 50 7 1125-1127

Areas of Advance

Nanoscience and Nanotechnology

Energy

Life Science Engineering

Materials Science

Subject Categories

Chemical Sciences

DOI

10.1021/bi1015887

More information

Created

10/6/2017