Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery
Journal article, 2011
Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic equilibrium. Moreover, dendrimer surface modification through PEGylation appears to make the interaction with DNA more reversible, which is favorable from the perspective of vector unpacking. Probing the nonequilibrium state of DNA during condensation processes is thus important for developing novel vectors, and further, it could also be useful in the study of chromatin folding.
starburst dendrimers
complexes
acetylation
pamam dendrimers
oligonucleotides
polyamidoamine dendrimers
efficiency
cytotoxicity
transfection
bromide
antisense
Author
Kristina Fant
Bengt Nordén
Per Lincoln
Biochemistry
0006-2960 (ISSN) 1520-4995 (eISSN)
Vol. 50 7 1125-1127Areas of Advance
Nanoscience and Nanotechnology
Energy
Life Science Engineering (2010-2018)
Materials Science
Subject Categories (SSIF 2011)
Chemical Sciences
DOI
10.1021/bi1015887