Using Ethidium To Probe Nonequilibrium States of DNA Condensed for Gene Delivery
Artikel i vetenskaplig tidskrift, 2011

Here we explore the use of ethidium to determine relative affinities of different gene delivery vectors for DNA and describe an improved method for studying the interaction. Specifically, we investigate the binding of poly(amidoamine) dendrimers and show that the DNA-dendrimer-ethidium system is far from thermodynamic equilibrium. Moreover, dendrimer surface modification through PEGylation appears to make the interaction with DNA more reversible, which is favorable from the perspective of vector unpacking. Probing the nonequilibrium state of DNA during condensation processes is thus important for developing novel vectors, and further, it could also be useful in the study of chromatin folding.

starburst dendrimers

complexes

acetylation

pamam dendrimers

oligonucleotides

polyamidoamine dendrimers

efficiency

cytotoxicity

transfection

bromide

antisense

Författare

Kristina Fant

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Bengt Nordén

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Per Lincoln

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Biochemistry

0006-2960 (ISSN) 1520-4995 (eISSN)

Vol. 50 1125-1127

Styrkeområden

Nanovetenskap och nanoteknik

Energi

Livsvetenskaper och teknik

Materialvetenskap

Ämneskategorier

Kemi

DOI

10.1021/bi1015887