Revealing the beneficial effect of protease supplementation to high gravity beer fermentations using "-omics" techniques
Journal article, 2011

Background: Addition of sugar syrups to the basic wort is a popular technique to achieve higher gravity in beer fermentations, but it results in dilution of the free amino nitrogen (FAN) content in the medium. The multicomponent protease enzyme Flavourzyme has beneficial effect on the brewer's yeast fermentation performance during high gravity fermentations as it increases the initial FAN value and results in higher FAN uptake, higher specific growth rate, higher ethanol yield and improved flavour profile. Results: In the present study, transcriptome and metabolome analysis were used to elucidate the effect on the addition of the multicomponent protease enzyme Flavourzyme and its influence on the metabolism of the brewer's yeast strain Weihenstephan 34/70. The study underlines the importance of sufficient nitrogen availability during the course of beer fermentation. The applied metabolome and transcriptome analysis allowed mapping the effect of the wort sugar composition on the nitrogen uptake. Conclusion: Both the transcriptome and the metabolome analysis revealed that there is a significantly higher impact of protease addition for maltose syrup supplemented fermentations, while addition of glucose syrup to increase the gravity in the wort resulted in increased glucose repression that lead to inhibition of amino acid uptake and hereby inhibited the effect of the protease addition.

yeast

probe level

pathway

amino

carbon catabolite repression

mass-spectrometry

saccharomyces-cerevisiae

Author

M. P. Piddocke

Technical University of Denmark (DTU)

A. Fazio

Technical University of Denmark (DTU)

Wanwipa Vongsangnak

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

M. L. Wong

Technical University of Denmark (DTU)

H. P. Heldt-Hansen

Novozymes A/S

C. T. Workman

Technical University of Denmark (DTU)

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Lisbeth Olsson

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Microbial Cell Factories

1475-2859 (ISSN)

Vol. 10 27

Subject Categories

Industrial Biotechnology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1186/1475-2859-10-27

More information

Latest update

7/3/2018 1