COORDINATION OF MULTIPLE DNA-MOLECULES IN RECA FIBER EVIDENCED BY LINEAR DICHROISM SPECTROSCOPY
Journal article, 1991
Polarized light spectroscopy has been used to study the interaction of RecA protein with DNA. Several different DNA complexes have been identified and characterized with respect to stoichiometries, base orientation and nuclease accessibility. By using spectroscopically distinguishable DNAs, we determined the number of DNA molecules co-ordinated by the RecA fiber in each of these complexes, and established their base pairing abilities. Based on these observations, we discuss the molecular mechanism of the RecA-mediated strand exchange reaction.
lexa repressor
recombination
protein-binding
escherichia-coli
electron-microscopy
genetic-recombination
reca-dna complex
lambda repressor
strand exchange
helical filaments
strand exchange
duplex dna
linear dichroism
reca
flow-oriented solution
Author
Masayuki Takahashi
Mikael Kubista
Department of Physical Chemistry
Bengt Nordén
Department of Physical Chemistry
Biochimie
0300-9084 (ISSN)
Vol. 73 2-3 219-226Subject Categories
Biochemistry and Molecular Biology
DOI
10.1016/0300-9084(91)90205-f