COORDINATION OF MULTIPLE DNA-MOLECULES IN RECA FIBER EVIDENCED BY LINEAR DICHROISM SPECTROSCOPY
Artikel i vetenskaplig tidskrift, 1991

Polarized light spectroscopy has been used to study the interaction of RecA protein with DNA. Several different DNA complexes have been identified and characterized with respect to stoichiometries, base orientation and nuclease accessibility. By using spectroscopically distinguishable DNAs, we determined the number of DNA molecules co-ordinated by the RecA fiber in each of these complexes, and established their base pairing abilities. Based on these observations, we discuss the molecular mechanism of the RecA-mediated strand exchange reaction.

lexa repressor

recombination

protein-binding

escherichia-coli

electron-microscopy

genetic-recombination

reca-dna complex

lambda repressor

strand exchange

helical filaments

strand exchange

duplex dna

linear dichroism

reca

flow-oriented solution

Författare

Masayuki Takahashi

Mikael Kubista

Institutionen för fysikalisk kemi

Bengt Nordén

Institutionen för fysikalisk kemi

Biochimie

0300-9084 (ISSN)

Vol. 73 2-3 219-226

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1016/0300-9084(91)90205-f