Bacterial cellulose as a potential vascular graft: Mechanical characterization and constitutive model development.
Journal article, 2011
Bacterial cellulose (BC) is a polysaccharide produced by Acetobacter Xylinum bacteria with interesting properties for arterial grafting and vascular tissue engineering including high-burst pressure, high-water content, high crystallinity, and an ultrafine highly pure fibrous structure similar to that of collagen. Given that compliance mismatch is one of the main factors contributing to the development of intimal hyperplasia in vascular replacement conduits, an in depth investigation of support mechanical properties of BC is required to further supporting its use in cardiovascular-grafting applications. The aim of this study was to mechanically characterize BC and also study its potential to accommodate vascular cells. To achieve these aims, inflation tests and uniaxial tensile tests were carried out on BC samples. In addition, dynamic compliance tests were conducted on BC tubes, and the results were compared to that of arteries, saphenous vein, expanded polytetrafluoroethylene, and Dacron grafts. BC tubes exhibited a compliance response similar to human saphenous vein with a mean compliance value of 4.27 × 10(-2) % per millimeter of mercury over the pressure range of 30-120 mmHg. In addition, bovine smooth muscle cells and endothelial cells were cultured on BC samples, and histology and fluorescent imaging analysis were carried out showing good adherence and biocompatibility. Finally, a method to predict the mechanical behavior of BC grafts in situ was established, whereby a constitutive model for BC was determined and used to model the BC tubes under inflation using finite element analysis.
Cattle
Blood Vessel Prosthesis
Cells
Polytetrafluoroethylene
chemistry
Polyethylene Terephthalates
chemistry
Endothelial Cells
Cultured
Models
chemistry
Biological
Animals
Arteries
Humans
chemistry
Saphenous Vein
Materials Testing
cytology
chemistry
Methylcellulose
Bioprosthesis
metabolism
Gluconacetobacter xylinus
chemistry
Author
H Zahedmanesh
Dublin City University
J N Mackle
Dublin City University
Anders Sellborn
University of Gothenburg
Kristoffer Drotz
Chalmers, Chemical and Biological Engineering, Polymer Technology
Aase Katarina Bodin
Chalmers, Chemical and Biological Engineering, Polymer Technology
Paul Gatenholm
Chalmers, Chemical and Biological Engineering, Polymer Technology
C Lally
Dublin City University
Journal of biomedical materials research. Part B, Applied biomaterials
1552-4981 (eISSN)
Vol. 97 1 105-13Subject Categories
MEDICAL AND HEALTH SCIENCES
DOI
10.1002/jbm.b.31791
PubMed
21290588