Bacterial cellulose as a potential vascular graft: Mechanical characterization and constitutive model development.
Artikel i vetenskaplig tidskrift, 2011

Bacterial cellulose (BC) is a polysaccharide produced by Acetobacter Xylinum bacteria with interesting properties for arterial grafting and vascular tissue engineering including high-burst pressure, high-water content, high crystallinity, and an ultrafine highly pure fibrous structure similar to that of collagen. Given that compliance mismatch is one of the main factors contributing to the development of intimal hyperplasia in vascular replacement conduits, an in depth investigation of support mechanical properties of BC is required to further supporting its use in cardiovascular-grafting applications. The aim of this study was to mechanically characterize BC and also study its potential to accommodate vascular cells. To achieve these aims, inflation tests and uniaxial tensile tests were carried out on BC samples. In addition, dynamic compliance tests were conducted on BC tubes, and the results were compared to that of arteries, saphenous vein, expanded polytetrafluoroethylene, and Dacron grafts. BC tubes exhibited a compliance response similar to human saphenous vein with a mean compliance value of 4.27 × 10(-2) % per millimeter of mercury over the pressure range of 30-120 mmHg. In addition, bovine smooth muscle cells and endothelial cells were cultured on BC samples, and histology and fluorescent imaging analysis were carried out showing good adherence and biocompatibility. Finally, a method to predict the mechanical behavior of BC grafts in situ was established, whereby a constitutive model for BC was determined and used to model the BC tubes under inflation using finite element analysis.

Cattle

Blood Vessel Prosthesis

Cells

Polytetrafluoroethylene

chemistry

Polyethylene Terephthalates

chemistry

Endothelial Cells

Cultured

Models

chemistry

Biological

Animals

Arteries

Humans

chemistry

Saphenous Vein

Materials Testing

cytology

chemistry

Methylcellulose

Bioprosthesis

metabolism

Gluconacetobacter xylinus

chemistry

Författare

H Zahedmanesh

Dublin City University

J N Mackle

Dublin City University

Anders Sellborn

Göteborgs universitet

Kristoffer Drotz

Chalmers, Kemi- och bioteknik, Polymerteknologi

Aase Katarina Bodin

Chalmers, Kemi- och bioteknik, Polymerteknologi

Paul Gatenholm

Chalmers, Kemi- och bioteknik, Polymerteknologi

C Lally

Dublin City University

Journal of biomedical materials research. Part B, Applied biomaterials

1552-4981 (eISSN)

Vol. 97 1 105-13

Ämneskategorier

MEDICIN OCH HÄLSOVETENSKAP

DOI

10.1002/jbm.b.31791

PubMed

21290588