Lipid biosynthesis monitored at the single-cell level in Saccharomyces cerevisiae
Journal article, 2011

There is increasing interest in bioengineering of lipids for use in functional foods, pharmaceuticals, and biofuels. Saccharomyces cerevisiae is a widely utilized cell factory for biotechnological production, thus a tempting alternative. Herein, we show how its neutral lipid accumulation varies throughout metabolic phases under nutritional conditions relevant for large-scale fermentation. Population-averaged metabolic data were correlated with lipid storage at the single-cell level monitored at submicron resolution by label-free coherent anti-Stokes Raman scattering (CARS) microscopy. While lipid droplet sizes are fairly constant, the number of droplets is a dynamic parameter determined by glucose and ethanol levels. The lowest number of lipid droplets is observed in the transition phase between glucose and ethanol fermentation. It is followed by a buildup during the ethanol phase. The surplus of accumulated lipids is then mobilized at concurrent glucose and ethanol starvation in the subsequent stationary phase. Thus, the highest amount of lipids is found in the ethanol phase, which is about 0.3 fL/cell. Our results indicate that the budding yeast, S. cerevisiae, can be used for the biosynthesis of lipids and demonstrate the strength of CARS microscopy for monitoring the dynamics of lipid metabolism at the single-cell level of importance for optimized lipid production.

Saccharomyces cerevisiae

Lipid droplet quantification

Nutrient Stress

CARS microscopy

Lipid storage

Author

Pramote Chumnanpuen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Christian Brackmann

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Subir Kumar Nandy

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Susana Chatzipapadopoulos

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Annika Enejder

Chalmers, Chemical and Biological Engineering, Molecular Imaging

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)

5 594-601

Subject Categories

Cell Biology

Biochemistry and Molecular Biology

Microbiology

Roots

Basic sciences

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1002/biot.201000386

More information

Created

10/8/2017