Lipid biosynthesis monitored at the single-cell level in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2011

There is increasing interest in bioengineering of lipids for use in functional foods, pharmaceuticals, and biofuels. Saccharomyces cerevisiae is a widely utilized cell factory for biotechnological production, thus a tempting alternative. Herein, we show how its neutral lipid accumulation varies throughout metabolic phases under nutritional conditions relevant for large-scale fermentation. Population-averaged metabolic data were correlated with lipid storage at the single-cell level monitored at submicron resolution by label-free coherent anti-Stokes Raman scattering (CARS) microscopy. While lipid droplet sizes are fairly constant, the number of droplets is a dynamic parameter determined by glucose and ethanol levels. The lowest number of lipid droplets is observed in the transition phase between glucose and ethanol fermentation. It is followed by a buildup during the ethanol phase. The surplus of accumulated lipids is then mobilized at concurrent glucose and ethanol starvation in the subsequent stationary phase. Thus, the highest amount of lipids is found in the ethanol phase, which is about 0.3 fL/cell. Our results indicate that the budding yeast, S. cerevisiae, can be used for the biosynthesis of lipids and demonstrate the strength of CARS microscopy for monitoring the dynamics of lipid metabolism at the single-cell level of importance for optimized lipid production.

Saccharomyces cerevisiae

Lipid droplet quantification

Nutrient Stress

CARS microscopy

Lipid storage


Pramote Chumnanpuen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Christian Brackmann

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Subir Kumar Nandy

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Susana Chatzipapadopoulos

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Jens B Nielsen

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Annika Enejder

Chalmers, Kemi- och bioteknik, Molekylär mikroskopi

Biotechnology journal

1860-6768 (ISSN) 1860-7314 (eISSN)




Biokemi och molekylärbiologi



Grundläggande vetenskaper


Livsvetenskaper och teknik