Integrated analysis of transcriptome and lipid profiling reveals the co-influences of inositol-choline and Snf1 in controlling lipid biosynthesis in yeast
Journal article, 2012

In the yeast Saccharomyces cerevisiae many genes involved in lipid biosynthesis are transcriptionally controlled by inositol-choline and the protein kinase Snf1. Here we undertook a global study on how inositol-choline and Snf1 interact in controlling lipid metabolism in yeast. Using both a reference strain (CEN.PK113-7D) and a snf1 Delta strain cultured at different nutrient limitations (carbon and nitrogen), at a fixed specific growth rate of 0.1 h(-1), and at different inositol choline concentrations, we quantified the expression of genes involved in lipid biosynthesis and the fluxes towards the different lipid components. Through integrated analysis of the transcriptome, the lipid profiling and the fluxome, it was possible to obtain a high quality, large-scale dataset that could be used to identify correlations and associations between the different components. At the transcription level, Snf1 and inositol-choline interact either directly through the main phospholipid-involving transcription factors (i.e. Ino2, Ino4, and Opi1) or through other transcription factors e.g. Gis1, Mga2, and Hac1. However, there seems to be flux regulation at the enzyme levels of several lipid involving enzymes. The analysis showed the strength of using both transcriptome and lipid profiling analysis for mapping the co-influence of inositol-choline and Snf1 on phospholipid metabolism.

kinase

microbial lipids

Lipid profiling

Inositol-choline

phospholipid biosynthesis

histone

saccharomyces-cerevisiae

gene-expression

phosphatidylserine synthase

c/n ratio

Transcriptome

ino1 promoter

Snf1

metabolism

Co-influence

protein-kinase

Author

Pramote Chumnanpuen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jie Zhang

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Intawat Nookaew

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Molecular Genetics and Genomics

1617-4615 (ISSN) 1617-4623 (eISSN)

Vol. 287 7 541-554

Subject Categories

Biochemistry and Molecular Biology

Areas of Advance

Life Science Engineering (2010-2018)

DOI

10.1007/s00438-012-0697-5

More information

Created

10/8/2017