Nanomechanotransduction and interphase nuclear organization influence on genomic control.
Journal article, 2007
The ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors.
chemistry
Centromere
Cell Nucleus
Silicon
Cellular
chemistry
Transformed
Polyethylenes
Quaternary Ammonium Compounds
genetics
Humans
metabolism
ultrastructure
metabolism
chemistry
Telomerase
chemistry
Oligonucleotide Array Sequence Analysis
Fibroblasts
Nanotechnology
Electroplating
Cell Culture Techniques
Mechanotransduction
Sulfonic Acids
ultrastructure
Genome
Substrate Specificity
chemistry
Cell Line
Water
Biocompatible
metabolism
metabolism
ultrastructure
metabolism
methods
Human
Biotin
Interphase
Nickel
chemistry
Propidium
chemistry
genetics
Coated Materials
Fluorescent Dyes
Fluorescein-5-isothiocyanate
genetics
Polymers
chemistry
Colloids
Polymethyl Methacrylate
chemistry
metabolism
genetics
metabolism
Author
Matthew J Dalby
Nikolaj Gadegaard
Pawel Herzyk
Duncan Sutherland
Hossein Agheli
Chalmers, Applied Physics, Chemical Physics
Chris D W Wilkinson
Adam S G Curtis
Journal of Cellular Biochemistry
0730-2312 (ISSN) 1097-4644 (eISSN)
Vol. 102 5 1234-44Subject Categories
Biological Sciences
DOI
10.1002/jcb.21354
PubMed
17427951