Nanomechanotransduction and interphase nuclear organization influence on genomic control.
Artikel i vetenskaplig tidskrift, 2007

The ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors.

chemistry

Centromere

Cell Nucleus

Silicon

Cellular

chemistry

Transformed

Polyethylenes

Quaternary Ammonium Compounds

genetics

Humans

metabolism

ultrastructure

metabolism

chemistry

Telomerase

chemistry

Oligonucleotide Array Sequence Analysis

Fibroblasts

Nanotechnology

Electroplating

Cell Culture Techniques

Mechanotransduction

Sulfonic Acids

ultrastructure

Genome

Substrate Specificity

chemistry

Cell Line

Water

Biocompatible

metabolism

metabolism

ultrastructure

metabolism

methods

Human

Biotin

Interphase

Nickel

chemistry

Propidium

chemistry

genetics

Coated Materials

Fluorescent Dyes

Fluorescein-5-isothiocyanate

genetics

Polymers

chemistry

Colloids

Polymethyl Methacrylate

chemistry

metabolism

genetics

metabolism

Författare

Matthew J Dalby

Nikolaj Gadegaard

Pawel Herzyk

Duncan Sutherland

Hossein Agheli

Chalmers, Teknisk fysik, Kemisk fysik

Chris D W Wilkinson

Adam S G Curtis

Journal of Cellular Biochemistry

0730-2312 (ISSN) 1097-4644 (eISSN)

Vol. 102 1234-44

Ämneskategorier

Biologiska vetenskaper

DOI

10.1002/jcb.21354

PubMed

17427951