Chitosan conjugates for DNA delivery
Journal article, 2013

Graft copolymers of chitosan and maltodextrin were synthesized by reductive amination of a low molecular weight chitosan. The degree of substitution is 70% or above, as quantified by nuclear magnetic resonance spectroscopy, at molar ratios of chitosan glucosamine units and maltodextrin of 1 : 1 and 1 : 5. The high substitution degree generates a water-soluble compound at pH 7.4 by reducing the amino groups of chitosan. In addition, the copolymer self-assembles into spherical nanoparticles with diameters of about 300 nm. The mass polymer/DNA ratios at the isoelectric point are within the range of 3-3.5 for chitosan-maltodextrin nanoparticles as determined by electrophoretic mobility measurements. These results confirm that DNA interacts efficiently with the copolymer nanoparticles indicating a potential application of the system for DNA delivery.

Author

D. Paiva

Universidade do Porto

G. Ivanova

Universidade do Porto

M. C. Pereira

Universidade do Porto

Sandra Rocha

Chalmers, Chemical and Biological Engineering, Industrial biotechnology

Physical Chemistry Chemical Physics

1463-9076 (ISSN) 1463-9084 (eISSN)

Vol. 15 28 11893-11899

Subject Categories

Chemical Engineering

DOI

10.1039/c3cp51215k

More information

Latest update

3/19/2018