Chitosan conjugates for DNA delivery
Artikel i vetenskaplig tidskrift, 2013

Graft copolymers of chitosan and maltodextrin were synthesized by reductive amination of a low molecular weight chitosan. The degree of substitution is 70% or above, as quantified by nuclear magnetic resonance spectroscopy, at molar ratios of chitosan glucosamine units and maltodextrin of 1 : 1 and 1 : 5. The high substitution degree generates a water-soluble compound at pH 7.4 by reducing the amino groups of chitosan. In addition, the copolymer self-assembles into spherical nanoparticles with diameters of about 300 nm. The mass polymer/DNA ratios at the isoelectric point are within the range of 3-3.5 for chitosan-maltodextrin nanoparticles as determined by electrophoretic mobility measurements. These results confirm that DNA interacts efficiently with the copolymer nanoparticles indicating a potential application of the system for DNA delivery.

Författare

D. Paiva

Universidade do Porto

G. Ivanova

Universidade do Porto

M. C. Pereira

Universidade do Porto

Sandra Rocha

Chalmers, Kemi- och bioteknik, Industriell Bioteknik

Physical Chemistry Chemical Physics

1463-9076 (ISSN) 1463-9084 (eISSN)

Vol. 15 11893-11899

Ämneskategorier

Kemiteknik

DOI

10.1039/c3cp51215k