A multifunctional pipette for localized drug administration to brain slices
Journal article, 2013

We have developed a superfusion method utilizing an open-volume microfluidic device for administration of pharmacologically active substances to selected areas in brain slices with high spatio-temporal resolution. The method consists of a hydrodynamically confined flow of the active chemical compound, which locally stimulates neurons in brain slices, applied in conjunction with electrophysiological recording techniques to analyze the response. The microfluidic device, which is a novel free-standing multifunctional pipette, allows diverse superfusion experiments, such as testing the effects of different concentrations of drugs or drug candidates on neurons in different cell layers with high positional accuracy, affecting only a small number of cells. We demonstrate herein the use of the method with electrophysiological recordings of pyramidal cells in hippocampal and prefrontal cortex brain slices from rats, determine the dependence of electric responses on the distance of the superfusion device from the recording site, document a multifold gain in solution exchange time as compared to whole slice perfusion, and show that the device is able to store and deliver up to four solutions in a series. Localized solution delivery by means of open-volume microfluidic technology also reduces reagent consumption and tissue culture expenses significantly, while allowing more data to be collected from a single tissue slice, thus reducing the number of laboratory animals to be sacrificed for a study. (C) 2013 Elsevier B.V. All rights reserved.

Microfluidic technology

IN-VITRO

METHYL-D-ASPARTATE

Prefrontal cortex

Glutamate

PREFRONTAL CORTEX

Brain slices

NEURONS

Perfusion

LTP

ANTAGONIST

RECEPTOR-MEDIATED NEUROTRANSMISSION

RESPONSES

CLOZAPINE

Hippocampus

Author

Aikeremu Ahemaiti

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Alar Ainla

Physical Chemistry

Gavin Jeffries

Physical Chemistry

Holger Wigström

University of Gothenburg

Owe Orwar

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Aldo Jesorka

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Kent Eric Jardemark

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Journal of Neuroscience Methods

0165-0270 (ISSN)

Vol. 219 2 292-296

Subject Categories

Neurosciences

DOI

10.1016/j.jneumeth.2013.08.012

More information

Latest update

1/17/2019