A multifunctional pipette for localized drug administration to brain slices
Artikel i vetenskaplig tidskrift, 2013

We have developed a superfusion method utilizing an open-volume microfluidic device for administration of pharmacologically active substances to selected areas in brain slices with high spatio-temporal resolution. The method consists of a hydrodynamically confined flow of the active chemical compound, which locally stimulates neurons in brain slices, applied in conjunction with electrophysiological recording techniques to analyze the response. The microfluidic device, which is a novel free-standing multifunctional pipette, allows diverse superfusion experiments, such as testing the effects of different concentrations of drugs or drug candidates on neurons in different cell layers with high positional accuracy, affecting only a small number of cells. We demonstrate herein the use of the method with electrophysiological recordings of pyramidal cells in hippocampal and prefrontal cortex brain slices from rats, determine the dependence of electric responses on the distance of the superfusion device from the recording site, document a multifold gain in solution exchange time as compared to whole slice perfusion, and show that the device is able to store and deliver up to four solutions in a series. Localized solution delivery by means of open-volume microfluidic technology also reduces reagent consumption and tissue culture expenses significantly, while allowing more data to be collected from a single tissue slice, thus reducing the number of laboratory animals to be sacrificed for a study. (C) 2013 Elsevier B.V. All rights reserved.

Brain slices

Microfluidic technology

IN-VITRO

Hippocampus

RESPONSES

Perfusion

METHYL-D-ASPARTATE

Prefrontal cortex

Glutamate

LTP

ANTAGONIST

RECEPTOR-MEDIATED NEUROTRANSMISSION

CLOZAPINE

NEURONS

PREFRONTAL CORTEX

Författare

Aikeremu Ahemaiti

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Alar Ainla

Chalmers University of Technology

Gavin Jeffries

Chalmers University of Technology

Holger Wigström

Göteborgs universitet

Owe Orwar

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Aldo Jesorka

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Kent Eric Jardemark

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

Journal of Neuroscience Methods

0165-0270 (ISSN)

Vol. 219 292-296

Ämneskategorier

Neurovetenskaper

DOI

10.1016/j.jneumeth.2013.08.012