Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery.
Journal article, 2013
The control of activity and localization of transcription factors is critical for appropriate transcriptional responses. In eukaryotes, signal transduction components such as mitogen-activated protein kinase (MAPK) shuttle into the nucleus to activate transcription. It is not known in detail how different amounts of nuclear MAPK over time affect the transcriptional response. In the present study, we aimed to address this issue by studying the high osmolarity glycerol (HOG) system in Saccharomyces cerevisiae. We employed a conditional osmotic system, which changes the period of the MAPK Hog1 signal independent of the initial stress level. We determined the dynamics of the Hog1 nuclear localization and cell volume by single-cell analysis in well-controlled microfluidics systems and compared the responses with the global transcriptional output of cell populations. We discovered that the onset of the initial transcriptional response correlates with the potential of cells for rapid adaptation; cells that are capable of recovering quickly initiate the transcriptional responses immediately, whereas cells that require longer time to adapt also respond later. This is reflected by Hog1 nuclear localization, Hog1 promoter association and the transcriptional response, but not Hog1 phosphorylation, suggesting that a presently uncharacterized rapid adaptive mechanism precedes the Hog1 nuclear response. Furthermore, we found that the period of Hog1 nuclear residence affects the amplitude of the transcriptional response rather than the spectrum of responsive genes.
Phosphorylation
metabolism
adverse effects
Genetic
Saccharomyces cerevisiae Proteins
metabolism
Cell Nucleus
Mutation
adverse effects
Transcription
metabolism
Adaptation
metabolism
Mitogen-Activated Protein Kinases
Glycerol
Protein Transport
Up-Regulation
metabolism
genetics
Stress
Saccharomyces cerevisiae
Post-Translational
Physiological
Hypertonic Solutions
genetics
genetics
Genetic
Glycerol-3-Phosphate Dehydrogenase (NAD+)
Kinetics
Physiological
Osmotic Pressure
genetics
Promoter Regions
Indicators and Reagents
Protein Processing
MAP Kinase Signaling System
Author
Cecilia Geijer
University of Gothenburg
Dagmara Medrala Klein
University of Gothenburg
Elzbieta Petelenz-Kurdziel
University of Gothenburg
Abraham Ericsson
University of Gothenburg
Maria Smedh
University of Gothenburg
Mikael Andersson
University of Gothenburg
Mattias Goksör
University of Gothenburg
Mariona Nadal-Ribelles
Francesc Posas
Marcus Krantz
University of Gothenburg
Bodil Nordlander
University of Gothenburg
Stefan Hohmann
University of Gothenburg
The FEBS journal
1742-4658 (ISSN)
Vol. 280 16 3854-67Subject Categories
Biological Sciences
DOI
10.1111/febs.12382
PubMed
23758973