Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery.
Artikel i vetenskaplig tidskrift, 2013

The control of activity and localization of transcription factors is critical for appropriate transcriptional responses. In eukaryotes, signal transduction components such as mitogen-activated protein kinase (MAPK) shuttle into the nucleus to activate transcription. It is not known in detail how different amounts of nuclear MAPK over time affect the transcriptional response. In the present study, we aimed to address this issue by studying the high osmolarity glycerol (HOG) system in Saccharomyces cerevisiae. We employed a conditional osmotic system, which changes the period of the MAPK Hog1 signal independent of the initial stress level. We determined the dynamics of the Hog1 nuclear localization and cell volume by single-cell analysis in well-controlled microfluidics systems and compared the responses with the global transcriptional output of cell populations. We discovered that the onset of the initial transcriptional response correlates with the potential of cells for rapid adaptation; cells that are capable of recovering quickly initiate the transcriptional responses immediately, whereas cells that require longer time to adapt also respond later. This is reflected by Hog1 nuclear localization, Hog1 promoter association and the transcriptional response, but not Hog1 phosphorylation, suggesting that a presently uncharacterized rapid adaptive mechanism precedes the Hog1 nuclear response. Furthermore, we found that the period of Hog1 nuclear residence affects the amplitude of the transcriptional response rather than the spectrum of responsive genes.

Phosphorylation

metabolism

adverse effects

Genetic

Saccharomyces cerevisiae Proteins

metabolism

Cell Nucleus

Mutation

adverse effects

Transcription

metabolism

Adaptation

metabolism

Mitogen-Activated Protein Kinases

Glycerol

Protein Transport

Up-Regulation

metabolism

genetics

Stress

Saccharomyces cerevisiae

Post-Translational

Physiological

Hypertonic Solutions

genetics

genetics

Genetic

Glycerol-3-Phosphate Dehydrogenase (NAD+)

Kinetics

Physiological

Osmotic Pressure

genetics

Promoter Regions

Indicators and Reagents

Protein Processing

MAP Kinase Signaling System

Författare

Cecilia Geijer

Göteborgs universitet

Dagmara Medrala Klein

Göteborgs universitet

Elzbieta Petelenz-Kurdziel

Göteborgs universitet

Abraham Ericsson

Göteborgs universitet

Maria Smedh

Göteborgs universitet

Mikael Andersson

Göteborgs universitet

Mattias Goksör

Göteborgs universitet

Mariona Nadal-Ribelles

Francesc Posas

Marcus Krantz

Göteborgs universitet

Bodil Nordlander

Göteborgs universitet

Stefan Hohmann

Göteborgs universitet

The FEBS journal

1742-4658 (ISSN)

Vol. 280 16 3854-67

Ämneskategorier

Biologiska vetenskaper

DOI

10.1111/febs.12382

PubMed

23758973