Stefan Hohmann

Single-molecule Slimfield microscopy for real-time dissection of protein-protein interactions, stoichiometry and mobility directly in live eukaryotic cells

Focus on resolution

Applying microfluidic systems to study effects of glucose at single-cell level

The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms

2017

The yeast osmostress response is carbon source dependent

2017

Transcription factor clusters regulate genes in eukaryotic cells

2017

Single-cell study links metabolism with nutrient signaling and reveals sources of variability

Editor's comment on "CRISPR/Cas9-mediated gene editing in human zygotes using Cas9 protein"

Time-Resolved Single Cell, Sub-Cellular Compartmentalized Proteomics, Combining Precise Microfluidics, Deconvolution and Ultrasensitive Single-Molecule Microscopy

Transcription factor clusters regulate gene expression in yeast Saccharomyces cerevisiae

3D millisecond tracking of single-molecule fluorescent protein translocation in eukaryotic cells

Single-Molecule Transcription Factor Dynamics in Saccharomyces Cerevisiae Glucose Sensing

Strategies for structuring interdisciplinary education in systems biology: An European perspective

2016

Systems Level Analysis of the Yeast Osmo-Stat

Nobel Yeast Research

The mitogen-activated protein kinase Slt2 modulates arsenite transport through the aquaglyceroporin Fps1.

Molecular communication: crosstalk between the Snf1 and other signaling pathways

2015

A nonlinear mixed effects approach for modeling the cell-to-cell variability of Mig1 dynamics in yeast.

An integrated view on a eukaryotic osmoregulation system

A fungicide-responsive kinase as a tool for synthetic cell fate regulation

The mammalian AMP-activated protein kinase complex mediates glucose regulation of gene expression in the yeast Saccharomyces cerevisiae

Glucose de-repression by yeast AMP-activated protein kinase SNF1 is controlled via at least two independent steps

Yeast reveals unexpected roles and regulatory features of aquaporins and aquaglyceroporins.

Bridging the gaps in systems biology

Yeast AMP-activated protein kinase monitors glucose concentration changes and absolute glucose levels

A novel chloroplast localized Rab GTPase protein CPRabA5e is involved in stress, development, thylakoid biogenesis and vesicle transport in Arabidopsis.

Differential Role of HAMP-like Linkers in Regulating the Functionality of the Group III Histidine Kinase DhNik1p

Rewiring yeast osmostress signalling through the MAPK network reveals essential and non-essential roles of Hog1 in osmoadaptation

Pheromone-Induced Morphogenesis Improves Osmoadaptation Capacity by Activating the HOG MAPK Pathway

2013

Quantitative Analysis of Glycerol Accumulation, Glycolysis and Growth under Hyper Osmotic Stress

Synthetic biology: lessons from engineering yeast MAPK signalling pathways.

Initiation of the transcriptional response to hyperosmotic shock correlates with the potential for volume recovery.

Osmostress-induced cell volume loss delays yeast hog1 signaling by limiting diffusion processes and by hog1-specific effects.

2012

Time course gene expression profiling of yeast spore germination reveals a network of transcription factors orchestrating the global response

Yeast Aquaglyceroporins Use the Transmembrane Core to Restrict Glycerol Transport

A framework for mapping, visualisation and automatic model creation of signal-transduction networks.

Fungal fludioxonil sensitivity is diminished by a constitutively active form of the group III histidine kinase

The genome of the xerotolerant mold Wallemia sebi reveals adaptations to osmotic stress and suggests cryptic sexual reproduction

Automated Ensemble Modeling with modelMaGe: Analyzing Feedback Mechanisms in the Sho1 Branch of the HOG Pathway

Efficient Construction of Homozygous Diploid Strains Identifies Genes Required for the Hyper-Filamentous Phenotype in Saccharomyces cerevisiae.

Distributed biological computation with multicellular engineered networks.

The Ashbya gossypiiEF-1α promoter of the ubiquitously used MX cassettes is toxic to Saccharomyces cerevisiae.

Systems biology of microorganisms. Preface.

Design, synthesis, and characterization of a highly effective Hog1 inhibitor: a powerful tool for analyzing MAP kinase signaling in yeast.

Quantification of cell volume changes upon hyperosmotic stress in Saccharomyces cerevisiae.

Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation.

Design, synthesis and characterization of a highly effective Hog1 inhibitor: a powerful tool for analyzing MAP kinase signalling in yeast

One hundred years of Molecular Genetics and Genomics: 100 years of extra-nuclear inheritance.

Gothenburg special issue: Molecules of life.

UNICELLSYS - Understanding the cell's functional organization

Saccharomyces cerevisiae Spore Germination

Design, synthesis and characterization of a highly effective Hog1 inhibitor: a powerful tool for analyzing MAP kinase signalling in yeast

Genetic screening for suppressor mutations of the hyper- or non-filamentous growth of the yeast osmotic signalling mutants

Functional analysis of the Ashbya gossypii Fps1 homolog

Short cut to 1,2,3-triazole-based p38 MAP kinase inhibitors via [3+2]-cycloaddition chemistry

Robustness and fragility in the yeast High Osmolarity Glycerol (HOG) signal transduction pathway

Highly selective, cell-permeable and fast-acting wild-type Hog1 inhibitors as tools for studying cellular function of kinases

Robustness and fragility in the high osmolarity glycerol (HOG) pathway in S. cerevisiae

Control of high osmolarity signalling in the yeast Saccharomyces cerevisiae.

Expression of the aquaporin AQY2 gene and morphological adaptations are negatively regulated through the osmoregulatory HOG pathway.

Expression of the yeast aquaporin Aqy2 affects cell surface properties under the control of osmoregulatory and morphogenic signalling pathways.

Systems Biology: Nobel Symposium 146.

Robustness and fragility in the yeast high osmolarity glycerol (HOG) signal-transduction pathway.

Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.

Robustness and fragility in the yeast High Osmolarity signal transduction pathway

Case study in systematic modelling: thiamine uptake in the yeast Saccharomyces cerevisiae.

Isc1p plays a key role in hydrogen peroxide resistance and chronological lifespan through modulation of iron levels and apoptosis.

Production, characterization and crystallization of the Plasmodium falciparum aquaporin.

How quantitative measures unravel design principles in multi-stage phosphorylation cascades.

Exploring the impact of osmoadaptation on glycolysis using time-varying response-coefficients

The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulation.

Robustness and fragility in the yeast High Osmolarity signal transduction pathway

Expression of the yeast aquaporin AQY2 gene is negatively regulated via Hog1, Kss1, and Sfl1

Robustness analysis of HOG pathway related genes in Saccharomyces cerevisiae

AtPTR3, a wound-induced peptide transporter needed for defence against virulent bacterial pathogens in Arabidopsis

Robustness analysis of HOG pathway related genes in budding yeast

Structural and functional analysis of the osmoregulated yeast aquaglyceroporin Fps1

Towards a structural and functional understanding of human aquaporin 9

Hog1-mediated Metabolic Adjustments Following Hyperosmotic Shock in the Yeast Saccharomyces cerevisiae

Structural and functional analysis of the osmoregulated yeast aquaglyceroporin Fps1.

[Systems biology can radically change health care. Basis for individualized prediction, prevention and treatment]

Revealing the secrets of dormancy and of survival during desiccation

Yeast osmoregulation

A microfluidic system in combination with optical tweezers for analyzing rapid and reversible cytological alterations in single cells upon environmental changes

Regulatory Mechanism of the Yeast Aquaporin AQY2 Expression Under Osmotic Condition.

Gis4, a new component of the ion homeostasis system in the yeast Saccharomyces cerevisiae

Comparative analysis of HOG pathway proteins to generate hypotheses for functional analysis

Yeast systems biology to unravel the network of life

Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae

Expression of heterologous aquaporins for functional analysis in Saccharomyces cerevisiae

A simple mathematical model of adaptation to high osmolarity in yeast

The Pep4p vacuolar proteinase contributes to the turnover of oxidized proteins but PEP4 overexpression is not sufficient to increase chronological lifespan in Saccharomyces cerevisiae

Pdc2 coordinates expression of the THI regulon in the yeast Saccharomyces cerevisiae

A micro-fluidic system for studies of stress response in single cells using optical tweezers

Comparative genomics of the HOG-signalling system in fungi

Role of Isc1p in oxidative stress resistance and ageing in yeast

Robustness analysis of HOG pathway genes in Saccharomyces cerevisiae

Conditional Osmotic Stress in Yeast. A system to study transport through aquaglyceroporins and osmostress signaling

The Yeast Systems Biology Network: mating communities

Engineering of a novel Saccharomyces cerevisiae wine strain with a respiratory phenotype at high external glucose concentrations

Modelling signalling pathways-A yeast approach

Integrative model of the response of yeast to osmotic shock

Report of an EU Projects Workshop on Systems Biology held in Brussels, Belgium on 8 December 2004

Using yeast to study transport and structure-function relationship in aquaglyceroporins

Role of Fps1 hydrophilic extensions and identification of residues controlling glycerol transport

Aquaporins in yeasts and filamentous fungi

The Saccharomyces cerevisiae aquaporin Aqy1 is involved in sporulation

Accumulation and release of the osmolyte glycerol is independent of the putative MIP channel Spac977.17p in Schizosaccharomyces pombe

Role of hexose transport in control of glycolytic flux in Saccharomyces cerevisiae

Transcriptional responses to glucose at different glycolytic rates in Saccharomyces cerevisiae

Identification of residues controlling transport through the yeast aquaglyceroporin Fps1 using a genetic screen

A Regulatory Domain in the C-terminal Extension of the Yeast Glycerol Channel Fps1p

Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock

Turgor restoration: the critical event in signal cessation of the HOG pathway

Role of Fps1 hydrophilic extensions and identification of residues controlling glycerol transport

Analysis of the VPS74 gene of Saccharomyces cerevisiae using DNA microarrays

Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.

Shutting the MAP off - and on again?

A Short Regulatory Domain Restricts Glycerol Transport through Yeast Fps1p

Ser3p (Yer081wp) and Ser33p (Yil074cp) are phosphoglycerate dehydrogenases in Saccharomyces cerevisiae

Downregulation of the HOG MAP kinase pathway during yeast osmoadaptation

A short regulatory domain restricts glycerol transport through yeast Fps1p

Gis4p is a novel component of ion homeostasis in the yeast Saccharomyces cerevisiae

The yeast glycerol 3-phosphatases Gpp1p and Gpp2p are required for glycerol biosynthesis and differentially involved in the cellular responses to osmotic, anaerobic, and oxidative stress.

Fps1p controls the accumulation and release of the compatible solute glycerol in yeast osmoregulation.

The two isoenzymes for yeast NAD+-dependent glycerol 3-phosphate dehydrogenase encoded by GPD1 and GPD2 have distinct roles in osmoadaptation and redox regulation.