The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms
Artikel i vetenskaplig tidskrift, 2017

A yeast Saccharomyces cerevisiae Snf1 kinase, an analog of mammalian AMPK, regulates glucose derepression of genes required for utilization of alternative carbon sources through the transcriptional repressor Mig1. It has been suggested that the Glc7-Reg1 phosphatase dephosphorylates Mig1. Here we report that Mig1 is dephosphorylated by Glc7-Reg1 in an apparently glucose-dependent mechanism but also by a mechanism independent of glucose and Glc7-Reg1. In addition to serine/threonine phosphatases another process including tyrosine phosphorylation seems crucial for Mig1 regulation. Taken together, Mig1 dephosphorylation appears to be controlled in a complex manner, in line with the importance for rapid and sensitive regulation upon altered glucose concentrations in the growth medium.

Phosphatases

Mig1

Glucose repression

Dephosphorylation

Inhibitors

Författare

Sviatlana Shashkova

University of York

Göteborgs universitet

Adam J.M. Wollman

University of York

Mark C. Leake

University of York

Stefan Hohmann

Chalmers, Biologi och bioteknik

FEMS Microbiology Letters

0378-1097 (ISSN)

Vol. 364 14

Ämneskategorier

Mikrobiologi

DOI

10.1093/femsle/fnx133