Robustness and fragility in the yeast High Osmolarity signal transduction pathway
Poster (konferens), 2008

Objective: The cellular signalling networks that integrate various environmental stimuli with information on cellular status must be robust to stimuli fluctuations as well as to stochastic differences in the amounts of signalling components. Here, we challenge the high osmolarity glycerol response (HOG) signal transduction pathway in the yeast Saccharomyces cerevisiae with systematic disturbances in components’ expression levels implemented by a “genetic tug-of-war”, or gTOW, methodology. Results: The disturbances were performed under various external perturbations, including pathway activation by osmotic shock. The resulting phenotypes in this particular study reflect a wide range of sensitivities, and disperse without any clear pattern over biochemical functions and pathway modules alike, with the most sensitive nodes being PBS2 and SSK1. Conclusions: Ideally, the obtained sensitivity profiles will allow us to impose parameter constraints. However, a more important aspect is the qualitative improvement of model structures, when local fragilities cannot be explained by the model structure. Surprisingly, the “neighboring” nodes HOG1 and SSK2 were affected to a much lesser extent, questioning our current understanding.

Författare

Marcus Krantz

Göteborgs universitet

Lars-Göran Ottosson

Göteborgs universitet

Doryaneh Ahmadpour

Göteborgs universitet

Jonas Warringer

Göteborgs universitet

Christian Waltermann

Bodil Nordlander

Göteborgs universitet

Anders Blomberg

Göteborgs universitet

Stefan Hohmann

Göteborgs universitet

Hiroaki Kitano

9th International Conference on Systems Biology (ICSB2008) proceedings, 9th International Conference on Systems Biology (ICSB2008), August 22-28, Gothenburg

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi