The Effect of Lithium on the Budding Yeast Saccharomyces cerevisiae upon Stress Adaptation
Artikel i vetenskaplig tidskrift, 2022
Lithium salts are used in the treatment of mood disorders, cancer, and Alzheimer’s disease. It has been shown to prolong life span in several phyla; however, not yet in budding yeast. In our study, we investigate the influence of lithium on yeast cells’ viability by characterizing protein aggregate formation, cell volume, and molecular crowding in the context of stress adaptation. While our data suggest a concentration-dependent growth inhibition caused by LiCl, we show an extended long-term survival rate as an effect of lithium addition upon glucose deprivation. We show that caloric restriction mitigates the negative impact of LiCl on cellular survival. Therefore, we suggest that lithium could affect glucose metabolism upon caloric restriction, which could explain the extended long-term survival observed in our study. We find furthermore that lithium chloride did not affect an immediate salt-induced Hsp104-dependent aggregate formation but cellular adaptation to H2 O2 and acute glucose starvation. We presume that different salt types and concentrations interfere with effective Hsp104 recruitment or its ATP-dependent disaggregase activity as a response to salt stress. This work provides novel details of Li+ effect on live eukaryotic cells which may also be applicable in further research on the treatment of cancer, Alzheimer’s, or other age-related diseases in humans.
Lithium
Yeast
Long-term sur-vival
Protein aggregation
Macromolecular crowding
Osmotic stress
Författare
Patrick Philipp Reith
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Göteborgs universitet
Chalmers, Biologi och bioteknik, Systembiologi
Svenja Braam
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Niek Welkenhuysen
Chalmers, Biologi och bioteknik, Systembiologi
Göteborgs universitet
Sarah Lecinski
University of York
Jack Shepherd
University of York
Chris Macdonald
University of York
Mark C. Leake
University of York
Stefan Hohmann
Sviatlana Shashkova
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Marija Cvijovic
Göteborgs universitet
Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik
Microorganisms
20762607 (eISSN)
Vol. 10 3 590Synthetic Circuits for Robust Orthogonal Production (SynCrop)
Europeiska kommissionen (EU) (EC/H2020/764591), 2018-01-01 -- 2021-12-31.
Ämneskategorier
Cellbiologi
Cell- och molekylärbiologi
Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
DOI
10.3390/microorganisms10030590
PubMed
35336166