Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.
Artikel i vetenskaplig tidskrift, 2004
The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction.
activation
exercise
metabolism
glycolysis
glucose repression
glycolytic gene-expression
hypoxia
respiration
human skeletal-muscle
pyruvate decarboxylase
hexose transport
growth
mechanisms
individual hexose transporters
signalling
Författare
Karin Otterstedt
Chalmers
Christer Larsson
Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik
R Bill
Aston University
Anders Ståhlberg
Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik
E Boles
Johann Wolfgang Goethe Universität Frankfurt am Main
Stefan Hohmann
Göteborgs universitet
Lena Gustafsson
Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik
EMBO Reports
1469-221X (ISSN) 1469-3178 (eISSN)
Vol. 5 5 532-537Ämneskategorier
Biokemi och molekylärbiologi
DOI
10.1038/sj.embor.7400132