Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.
Artikel i vetenskaplig tidskrift, 2004

The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction.

glycolysis

human skeletal-muscle

hexose transport

respiration

metabolism

activation

pyruvate decarboxylase

individual hexose transporters

mechanisms

glycolytic gene-expression

glucose repression

growth

exercise

signalling

hypoxia

Författare

Karin Otterstedt

Chalmers University of Technology

Christer Larsson

Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik

R Bill

Aston University

Anders Ståhlberg

Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik

E Boles

Johann Wolfgang Goethe Universität Frankfurt am Main

Stefan Hohmann

Göteborgs universitet

Lena Gustafsson

Chalmers, Institutionen för kemi och biovetenskap, Molekylär bioteknik

EMBO Reports

1469-221X (ISSN) 1469-3178 (eISSN)

Vol. 5 532-537

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1038/sj.embor.7400132