Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.
Journal article, 2004
The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction.
activation
exercise
metabolism
glycolysis
glucose repression
glycolytic gene-expression
hypoxia
respiration
human skeletal-muscle
pyruvate decarboxylase
hexose transport
growth
mechanisms
individual hexose transporters
signalling
Author
Karin Otterstedt
Chalmers
Christer Larsson
Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology
R Bill
Aston University
Anders Ståhlberg
Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology
E Boles
Goethe University Frankfurt
Stefan Hohmann
University of Gothenburg
Lena Gustafsson
Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology
EMBO Reports
1469-221X (ISSN) 1469-3178 (eISSN)
Vol. 5 5 532-537Subject Categories
Biochemistry and Molecular Biology
DOI
10.1038/sj.embor.7400132