Switching the mode of metabolism in the yeast Saccharomyces cerevisiae.
Journal article, 2004

The biochemistry of most metabolic pathways is conserved from bacteria to humans, although the control mechanisms are adapted to the needs of each cell type. Oxygen depletion commonly controls the switch from respiration to fermentation. However, Saccharomyces cerevisiae also controls that switch in response to the external glucose level. We have generated an S. cerevisiae strain in which glucose uptake is dependent on a chimeric hexose transporter mediating reduced sugar uptake. This strain shows a fully respiratory metabolism also at high glucose levels as seen for aerobic organisms, and switches to fermentation only when oxygen is lacking. These observations illustrate that manipulating a single step can alter the mode of metabolism. The novel yeast strain is an excellent tool to study the mechanisms underlying glucose-induced signal transduction.

activation

exercise

metabolism

glycolysis

glucose repression

glycolytic gene-expression

hypoxia

respiration

human skeletal-muscle

pyruvate decarboxylase

hexose transport

growth

mechanisms

individual hexose transporters

signalling

Author

Karin Otterstedt

Chalmers

Christer Larsson

Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology

R Bill

Aston University

Anders Ståhlberg

Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology

E Boles

Goethe University Frankfurt

Stefan Hohmann

University of Gothenburg

Lena Gustafsson

Chalmers, Department of Chemistry and Bioscience, Molecular Biotechnology

EMBO Reports

1469-221X (ISSN) 1469-3178 (eISSN)

Vol. 5 5 532-537

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1038/sj.embor.7400132

More information

Latest update

9/10/2018