Genome-wide expression profile of the mnn2Δ mutant of Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2006

Abstract The MNN2 gene of S. cerevisiae encodes an α (1,2) mannosyl transferase required for branching the outer chain of N-linked oligosaccharides (Rayner J.C. and Munro S. 1998. J. Biol. Chem. 273: 26836–26843) and it also seems to have some effect on the transfer of mannosyl phosphate groups to the inner core (Olivero I. et al. 2000. FEBS Lett. 475: 111–116). In order to reveal possible interactions of MNN2 expression with other cellular pathways, we analyzed the transcriptome of the deletion mutant S. cerevisiae mnn2Δ using cDNA microarrays. We found 151 genes that showed an altered expression level of ≥2-fold, 58 of them up-regulated and 93 down-regulated. Quite a high proportion of these genes (29%) encode unclassified proteins. In contrast to other defects affecting the integrity of the cell wall, deletion of MNN2 does not stimulate the expression of any of the genes included in the previously defined ‘cell wall compensatory cluster’ (Lagorce et al. 2003. J. Biol. Chem. 278: 20345–20357). We also found that 15% of the selected genes are related to central metabolic pathways. In addition, the mnn2Δ strain seems to have a certain level of stimulation of DNA processing reactions while some genes involved in intracellular transport pathways are under-regulated

Mannoprotein

Cell wall

Saccharomyces cerevisiae

DNA microarrays

MNN2

N-glycosylation

Mannosyl phosphorylation

Författare

Isaac Corbacho

Isabel Olivero

Stefan Hohmann

Göteborgs universitet

Per Sunnerhagen

Göteborgs universitet

Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

0003-6072 (ISSN) 1572-9699 (eISSN)

Vol. 89 485-494

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1007/s10482-005-9047-5