A novel yeast hybrid modeling framework integrating Boolean and enzyme-constrained networks enables exploration of the interplay between signaling and metabolism
Artikel i vetenskaplig tidskrift, 2021

The interplay between nutrient-induced signaling and metabolism plays an important role in maintaining homeostasis and its malfunction has been implicated in many different human diseases such as obesity, type 2 diabetes, cancer, and neurological disorders. Therefore, unraveling the role of nutrients as signaling molecules and metabolites together with their interconnectivity may provide a deeper understanding of how these conditions occur. Both signaling and metabolism have been extensively studied using various systems biology approaches. However, they are mainly studied individually and in addition, current models lack both the complexity of the dynamics and the effects of the crosstalk in the signaling system. To gain a better understanding of the interconnectivity between nutrient signaling and metabolism in yeast cells, we developed a hybrid model, combining a Boolean module, describing the main pathways of glucose and nitrogen signaling, and an enzyme-constrained model accounting for the central carbon metabolism of Saccharomyces cerevisiae, using a regulatory network as a link. The resulting hybrid model was able to capture a diverse utalization of isoenzymes and to our knowledge outperforms constraint-based models in the prediction of individual enzymes for both respiratory and mixed metabolism. The model showed that during fermentation, enzyme utilization has a major contribution in governing protein allocation, while in low glucose conditions robustness and control are prioritized. In addition, the model was capable of reproducing the regulatory effects that are associated with the Crabtree effect and glucose repression, as well as regulatory effects associated with lifespan increase during caloric restriction. Overall, we show that our hybrid model provides a comprehensive framework for the study of the non-trivial effects of the interplay between signaling and metabolism, suggesting connections between the Snf1 signaling pathways and processes that have been related to chronological lifespan of yeast cells.


Linnea Österberg

Göteborgs universitet

Chalmers, Biologi och bioteknik, Systembiologi

Iván Domenzain Del Castillo Cerecer

Chalmers, Biologi och bioteknik, Systembiologi

Julia Münch

Chalmers, Matematiska vetenskaper

Göteborgs universitet

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

BioInnovation Institute

Stefan Hohmann

Chalmers, Biologi och bioteknik

Marija Cvijovic

Göteborgs universitet

Chalmers, Matematiska vetenskaper, Tillämpad matematik och statistik

PLoS Computational Biology

1553-734X (ISSN) 1553-7358 (eISSN)

Vol. 17 4 e1008891

Integration av metabolism och signalöverföring: den AMP-aktivierade protein kinas SNF1 i jäst

Vetenskapsrådet (VR) (2016-03744), 2017-01-01 -- 2021-12-31.

Model-Based Construction And Optimisation Of Versatile Chassis Yeast Strains For Production Of Valuable Lipid And Aromatic Compounds (CHASSY)

Europeiska kommissionen (EU) (EC/H2020/720824), 2016-12-01 -- 2020-11-30.


Farmaceutisk vetenskap


Bioinformatik (beräkningsbiologi)





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