Applying microfluidic systems to study effects of glucose at single-cell level
Artikel i vetenskaplig tidskrift, 2018

Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.

Microfluidic system

Fluorescence microscopy

Single cell analysis

Optical tweezers

Glucose uptake

Saccharomyces cerevisiae

Microbiology

Författare

Niek Welkenhuysen

Goteborgs Universitet

Caroline B. Adiels

Goteborgs Universitet

Mattias Goksör

Goteborgs Universitet

Stefan Hohmann

Chalmers, Biologi och bioteknik

Methods in molecular biology (Clifton, N.J.)

1064-3745 (ISSN)

Vol. 1713 109-121

Ämneskategorier

Biokemi och molekylärbiologi

DOI

10.1007/978-1-4939-7507-5_9