Applying microfluidic systems to study effects of glucose at single-cell level
Journal article, 2018

Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.

Glucose uptake

Single cell analysis

Microbiology

Optical tweezers

Saccharomyces cerevisiae

Fluorescence microscopy

Microfluidic system

Author

Niek Welkenhuysen

University of Gothenburg

Caroline B. Adiels

University of Gothenburg

Mattias Goksör

University of Gothenburg

Stefan Hohmann

Chalmers, Biology and Biological Engineering

Methods in Molecular Biology

10643745 (ISSN)

Vol. 1713 109-121

Subject Categories

Biochemistry and Molecular Biology

DOI

10.1007/978-1-4939-7507-5_9

More information

Latest update

2/21/2018