Robustness analysis of HOG pathway related genes in budding yeast
Poster (konferens), 2007

We applied a novel genetic screening method, named “genetic tug-of-war” (gTOW) to estimate the upper limit of gene copy numbers in Saccharomyces cerevisiae. The study involved 29 HOG pathway related genes which included kinases, phosphatases and transcription factors to cover different set of players in the signal transduction system. In addition a phenotypic profiling was conducted in four different growth conditions with three outputs: lag phase, growth phase and efficiency of growth. A number of interesting hits were identified, including PBS2 which had low numbers of gene copies. It will be of interest to expand the study to encompass the entire known signal transduction system in yeast to search for sensitive nodes.

Författare

Lars-Göran Ottosson

Göteborgs universitet

Doryaneh Ahmadpour

Göteborgs universitet

Marcus Krantz

Göteborgs universitet

Jonas Warringer

Göteborgs universitet

Anders Blomberg

Göteborgs universitet

Hiroaki Kitano

Stefan Hohmann

Göteborgs universitet

ICSB 2007 Conference Proceedings, 8th International Conference on Systems Biology (ICSB2007), October 1-6, 2007

Ämneskategorier

Cellbiologi

Biokemi och molekylärbiologi