Probing Physical Properties of a DNA- Protein Complex Using Nanofluidic Channels
Journal article, 2014

A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces. © 2014 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.

Author

Karolin Frykholm

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Mohammadreza Alizadehheidari

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Joachim Fritzsche

Chalmers, Applied Physics, Chemical Physics

Jens Wigenius

Chalmers, Chemical and Biological Engineering, Physical Chemistry

M. Modesti

Centre national de la recherche scientifique (CNRS)

Institut Paoli-Calmettes

Inserm Transfert

F. Persson

Uppsala University

Inserm Transfert

Fredrik Westerlund

Chalmers, Chemical and Biological Engineering, Physical Chemistry

Small

1613-6810 (ISSN) 1613-6829 (eISSN)

Vol. 10 5 884-887

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Biophysics

DOI

10.1002/smll.201302028

More information

Latest update

9/7/2018 1