Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics
Journal article, 2014
Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody-based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to approximate to 80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.
Author
L. Fagerberg
B. M. Hallstrom
P. Oksvold
C. Kampf
D. Djureinovic
J. Odeberg
M. Habuka
S. Tahmasebpoor
A. Danielsson
K. Edlund
A. Asplund
E. Sjostedt
E. Lundberg
C. A. Szigyarto
M. Skogs
J. O. Takanen
H. Berling
H. Tegel
J. Mulder
P. Nilsson
J. M. Schwenk
C. Lindskog
F. Danielsson
Adil Mardinoglu
Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology
A. Sivertsson
K. von Feilitzen
M. Forsberg
M. Zwahlen
I. Olsson
S. Navani
M. Huss
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology
F. Ponten
M. Uhlen
Molecular and Cellular Proteomics
1535-9476 (ISSN) 1535-9484 (eISSN)
Vol. 13 2 397-406Areas of Advance
Life Science Engineering (2010-2018)
Subject Categories
Chemical Sciences
DOI
10.1074/mcp.M113.035600