Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC degrees)
Journal article, 2014

We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA. © 2014 Elsevier B.V.

Author

F. Sellrie

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

C. Lenz

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

A. Andersson

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

Marcus Wilhelmsson

Chalmers, Chemical and Biological Engineering, Physical Chemistry

J. A. Schenk

Hybrotec GmbH

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

Talanta

0039-9140 (ISSN)

Vol. 124 67-70

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Physical Chemistry

Analytical Chemistry

Other Basic Medicine

Roots

Basic sciences

DOI

10.1016/j.talanta.2014.02.046

More information

Created

10/7/2017