Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC degrees)
Artikel i vetenskaplig tidskrift, 2014
We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA. © 2014 Elsevier B.V.
Författare
F. Sellrie
Fraunhofer-Institut fur Biomedizinische Technik (IBMT)
C. Lenz
Fraunhofer-Institut fur Biomedizinische Technik (IBMT)
A. Andersson
Fraunhofer-Institut fur Biomedizinische Technik (IBMT)
Marcus Wilhelmsson
Chalmers, Kemi- och bioteknik, Fysikalisk kemi
J. A. Schenk
Hybrotec GmbH
Fraunhofer-Institut fur Biomedizinische Technik (IBMT)
Talanta
0039-9140 (ISSN)
Vol. 124 67-70Styrkeområden
Nanovetenskap och nanoteknik
Livsvetenskaper och teknik (2010-2018)
Ämneskategorier
Fysikalisk kemi
Analytisk kemi
Annan medicinsk grundvetenskap
Fundament
Grundläggande vetenskaper
DOI
10.1016/j.talanta.2014.02.046