Antibody mediated fluorescence enhancement of nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC degrees)
Artikel i vetenskaplig tidskrift, 2014

We report on the generation and analytical application of the monoclonal antibody G93-ED2 raised against the tricyclic fluorescent nucleoside analogue 1,3-diaza-2-oxophenoxazine (tC). G93-ED2 is specifically binding this deoxycytidine analogue and was found to raise its fluorescence intensity by a factor of 5. This unique feature makes it a valuable tool in fluorescence dependent immunoassays. G93-ED2 was successfully applied in a homogeneous fluorescence quenching immunoassay (DNA-Q) for the sequence specific determination of DNA. © 2014 Elsevier B.V.

Författare

F. Sellrie

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

C. Lenz

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

A. Andersson

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

Marcus Wilhelmsson

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

J. A. Schenk

Hybrotec GmbH

Fraunhofer-Institut fur Biomedizinische Technik - IBMT

Talanta

0039-9140 (ISSN)

Vol. 124 67-70

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Fysikalisk kemi

Analytisk kemi

Annan medicinsk grundvetenskap

Fundament

Grundläggande vetenskaper

DOI

10.1016/j.talanta.2014.02.046

Mer information

Skapat

2017-10-07