Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
Journal article, 2014
AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.
RAUCH MA
1997
1995
V179
MOLECULAR MICROBIOLOGY
V177
TRANSITION-STATE REGULATOR
DOMAIN
V246
APRE EXPRESSION
EMBO JOURNAL
eilly M
P522
RAUCH MA
TRANSCRIPTION
P1203
P1615
V8
TYROSINE
PROMOTER
RAUCH MA
JOURNAL OF BACTERIOLOGY
MOLECULAR & GENERAL GENETICS
JOURNAL OF BACTERIOLOGY
RAUCH MA
V3
1995
1989
SPORULATION
SPECIFICITY
P756
P6999
1989
MYCOBACTERIUM-TUBERCULOSIS
PROTEIN-KINASES
Author
A. Kobir
Microbiologie de l'Alimentation au Service de la Sante Humaine
S. Poncet
Microbiologie de l'Alimentation au Service de la Sante Humaine
V. Bidnenko
Microbiologie de l'Alimentation au Service de la Sante Humaine
O. Delumeau
Microbiologie de l'Alimentation au Service de la Sante Humaine
C. Jers
Microbiologie de l'Alimentation au Service de la Sante Humaine
S. Zouhir
Centre national de la recherche scientifique (CNRS)
R. Grenha
Centre national de la recherche scientifique (CNRS)
S. Nessler
Centre national de la recherche scientifique (CNRS)
University of Paris-Sud
P. Noirot
Microbiologie de l'Alimentation au Service de la Sante Humaine
Ivan Mijakovic
Chalmers, Chemical and Biological Engineering, Life Sciences
Molecular Microbiology
0950-382X (ISSN) 1365-2958 (eISSN)
Vol. 92 5 1129-1141Subject Categories
Biological Systematics
DOI
10.1111/mmi.12617