Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
Artikel i vetenskaplig tidskrift, 2014

AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.

V3

P1203

RAUCH MA

V179

JOURNAL OF BACTERIOLOGY

TRANSCRIPTION

EMBO JOURNAL

1989

1997

PROMOTER

SPORULATION

eilly M

MYCOBACTERIUM-TUBERCULOSIS

P756

APRE EXPRESSION

RAUCH MA

1995

SPECIFICITY

JOURNAL OF BACTERIOLOGY

P6999

DOMAIN

V177

P1615

TRANSITION-STATE REGULATOR

V246

MOLECULAR MICROBIOLOGY

PROTEIN-KINASES

1989

TYROSINE

P522

RAUCH MA

RAUCH MA

MOLECULAR & GENERAL GENETICS

1995

V8

Författare

A. Kobir

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Poncet

Microbiologie de l'Alimentation au Service de la Sante Humaine

V. Bidnenko

Microbiologie de l'Alimentation au Service de la Sante Humaine

O. Delumeau

Microbiologie de l'Alimentation au Service de la Sante Humaine

C. Jers

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Zouhir

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

R. Grenha

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

S. Nessler

Université Paris-Sud

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

P. Noirot

Microbiologie de l'Alimentation au Service de la Sante Humaine

Ivan Mijakovic

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Molecular Microbiology

0950-382X (ISSN) 1365-2958 (eISSN)

Vol. 92 5 1129-1141

Ämneskategorier

Biologisk systematik

DOI

10.1111/mmi.12617