Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
Artikel i vetenskaplig tidskrift, 2014

AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.

1989

SPORULATION

SPECIFICITY

RAUCH MA

V246

TRANSITION-STATE REGULATOR

P1615

eilly M

P1203

1995

MYCOBACTERIUM-TUBERCULOSIS

RAUCH MA

P6999

DOMAIN

RAUCH MA

TYROSINE

V177

P522

1995

PROMOTER

V8

V179

JOURNAL OF BACTERIOLOGY

RAUCH MA

APRE EXPRESSION

1989

PROTEIN-KINASES

TRANSCRIPTION

MOLECULAR MICROBIOLOGY

V3

P756

1997

JOURNAL OF BACTERIOLOGY

EMBO JOURNAL

MOLECULAR & GENERAL GENETICS

Författare

A. Kobir

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Poncet

Microbiologie de l'Alimentation au Service de la Sante Humaine

V. Bidnenko

Microbiologie de l'Alimentation au Service de la Sante Humaine

O. Delumeau

Microbiologie de l'Alimentation au Service de la Sante Humaine

C. Jers

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Zouhir

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

R. Grenha

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

S. Nessler

Universite Paris-Sud XI

LEBS Laboratoire d'Enzymologie et Biochimie Structurales CNRS UPR 9063

P. Noirot

Microbiologie de l'Alimentation au Service de la Sante Humaine

Ivan Mijakovic

Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Molecular Microbiology

0950-382X (ISSN) 1365-2958 (eISSN)

Vol. 92 1129-1141

Ämneskategorier

Biologisk systematik

DOI

10.1111/mmi.12617