Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties
Artikel i vetenskaplig tidskrift, 2014

AbrB is a global gene regulator involved in transition phase phenomena in Bacillus subtilis. It participates in a complex regulatory network governing the expression of stationary-phase functions. AbrB was previously found to be phosphorylated on serine 86 located close to its C-terminal oligomerization domain. Here we report that AbrB can be phosphorylated by three B. subtilis serine/threonine kinases expressed during the transition and stationary phase: PrkC, PrkD and YabT. Our in vitro findings suggest that AbrB phosphorylation impedes its DNA binding and abolishes binding cooperativity. In vivo we established that a phospho-mimetic mutation abrB S86D leads to a significant loss of AbrB control over several key target functions: exoprotease production, competence development and sporulation. A wider transcriptome analysis of abrBS86D and S86A mutant strains revealed deregulation of a large number of target genes. We therefore propose that AbrB phosphorylation serves as an additional input for fine-tuning the activity of this ambiactive gene regulator.

RAUCH MA

1997

1995

V179

MOLECULAR MICROBIOLOGY

V177

TRANSITION-STATE REGULATOR

DOMAIN

V246

APRE EXPRESSION

EMBO JOURNAL

eilly M

P522

RAUCH MA

TRANSCRIPTION

P1203

P1615

V8

TYROSINE

PROMOTER

RAUCH MA

JOURNAL OF BACTERIOLOGY

MOLECULAR & GENERAL GENETICS

JOURNAL OF BACTERIOLOGY

RAUCH MA

V3

1995

1989

SPORULATION

SPECIFICITY

P756

P6999

1989

MYCOBACTERIUM-TUBERCULOSIS

PROTEIN-KINASES

Författare

A. Kobir

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Poncet

Microbiologie de l'Alimentation au Service de la Sante Humaine

V. Bidnenko

Microbiologie de l'Alimentation au Service de la Sante Humaine

O. Delumeau

Microbiologie de l'Alimentation au Service de la Sante Humaine

C. Jers

Microbiologie de l'Alimentation au Service de la Sante Humaine

S. Zouhir

Centre national de la recherche scientifique (CNRS)

R. Grenha

Centre national de la recherche scientifique (CNRS)

S. Nessler

Centre national de la recherche scientifique (CNRS)

Université Paris-Sud

P. Noirot

Microbiologie de l'Alimentation au Service de la Sante Humaine

Ivan Mijakovic

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Molecular Microbiology

0950-382X (ISSN) 1365-2958 (eISSN)

Vol. 92 5 1129-1141

Ämneskategorier

Biologisk systematik

DOI

10.1111/mmi.12617

Mer information

Senast uppdaterat

2018-09-07