Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae
Journal article, 2014

Background: The koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome. Results: Here we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three a-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase. Conclusion: In combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.

ENDOPLASMIC-RETICULUM

Aspergillus oryzae

Comparative genomics

SACCHAROMYCES-CEREVISIAE

AMYLASE GENES

Mathematical & Computational Biology

1988

Transcriptome

CELL-WALLS

V6

STRESS-RESPONSE

alpha-amylase

GENE-EXPRESSION

INDUCTION

Secretory pathway

P1419

FILAMENTOUS FUNGI

NIGER

BIO-TECHNOLOGY

RISTENSEN T

TRANSCRIPTIONAL ACTIVATOR

Author

Lifang Liu

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Amir Feizi

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

Tobias Österlund

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

C. Hjort

Novozymes A/S

Jens B Nielsen

Chalmers, Chemical and Biological Engineering, Life Sciences, System Biology

BMC Systems Biology

1752-0509 (ISSN)

Vol. 8 73 73

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

European Commission (FP7), 2010-01-01 -- 2014-12-31.

Infrastructure

C3SE (Chalmers Centre for Computational Science and Engineering)

Areas of Advance

Life Science Engineering (2010-2018)

Subject Categories

Bioinformatics and Systems Biology

DOI

10.1186/1752-0509-8-73

More information

Latest update

7/3/2018 1