Identifying bacteria using DNA binding maps
Paper in proceedings, 2013

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Nanofluidics

Optical mapping

Bacterial identification

Author

Gustav Emilsson

Chalmers, Applied Physics, Bionanophotonics

A. Nilsson

Lund University

Lena Nyberg

Chemical Biology

C. Noble

Lund University

L. Svensson Stadler

University of Gothenburg

Edward R.B. Moore

University of Gothenburg

T. Ambjornsson

Lund University

J. O. Tegenfeldt

Lund University

Fredrik Westerlund

Chemical Biology

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 473-475

Areas of Advance

Nanoscience and Nanotechnology (2010-2017)

Life Science Engineering (2010-2018)

Subject Categories

Physical Chemistry

Nano Technology

More information

Latest update

11/26/2019