Identifying bacteria using DNA binding maps
Paper i proceeding, 2013

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Nanofluidics

Optical mapping

Bacterial identification

Författare

Gustav Emilsson

Chalmers, Teknisk fysik, Bionanofotonik

A. Nilsson

Lunds universitet

Lena Nyberg

Kemisk biologi

C. Noble

Lunds universitet

L. Svensson Stadler

Göteborgs universitet

Edward R.B. Moore

Göteborgs universitet

T. Ambjornsson

Lunds universitet

J. O. Tegenfeldt

Lunds universitet

Fredrik Westerlund

Kemisk biologi

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 473-475

Styrkeområden

Nanovetenskap och nanoteknik (2010-2017)

Livsvetenskaper och teknik (2010-2018)

Ämneskategorier

Fysikalisk kemi

Nanoteknik

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Senast uppdaterat

2019-11-26