Identifying bacteria using DNA binding maps
Paper i proceeding, 2014

We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.

Bacterial identification

Optical mapping

Nanofluidics

Författare

Gustav Emilsson

Chalmers, Teknisk fysik, Bionanofotonik

A. Nilsson

Lena Nyberg

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

C. Noble

L. Svensson Stadler

Edward R.B. Moore

T. Ambjornsson

J. O. Tegenfeldt

Fredrik Westerlund

Chalmers, Kemi- och bioteknik, Fysikalisk kemi

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013

Vol. 1 473-475

Styrkeområden

Nanovetenskap och nanoteknik

Livsvetenskaper och teknik

Ämneskategorier

Fysikalisk kemi

Nanoteknik

ISBN

978-163266624-6