Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Journal article, 2014
We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.
Biochemical Research Methods
FREQUENCY VIBRATIONAL-MODES
STRUCTURAL DYNAMICS
Author
David Arnlund
University of Gothenburg
Linda C Johansson
University of Gothenburg
Cecilia Wickstrand
University of Gothenburg
A. Barty
G. J. Williams
Erik Malmerberg
University of Gothenburg
J Davidsson
D. Milathianaki
D. P. Deponte
R. L. Shoeman
D. J. Wang
D. James
Gergely Katona
University of Gothenburg
Sebastian Westenhoff
University of Gothenburg
T. A. White
A. Aquila
S. Bari
Peter Berntsen
University of Gothenburg
M. Bogan
T. B. van Driel
R. B. Doak
K. S. Kjaer
M. Frank
R. Fromme
I. Grotjohann
R. Henning
M. S. Hunter
R. A. Kirian
I. Kosheleva
C. Kupitz
M. N. Liang
A. V. Martin
M. M. Nielsen
M. Messerschmidt
M. M. Seibert
Jennie Sjöhamn
University of Gothenburg
F. Stellato
U. Weierstall
N. A. Zatsepin
J. C. H. Spence
P. Fromme
I. Schlichting
S. Boutet
G. Groenhof
H. N. Chapman
Richard Neutze
University of Gothenburg
Nature Methods
1548-7091 (ISSN) 1548-7105 (eISSN)
Vol. 11 9 923-926Subject Categories
Chemical Sciences
DOI
10.1038/nmeth.3067