Visualizing a protein quake with time-resolved X-ray scattering at a free-electron laser
Artikel i vetenskaplig tidskrift, 2014

We describe a method to measure ultrafast protein structural changes using time-resolved wide-angle X-ray scattering at an X-ray free-electron laser. We demonstrated this approach using multiphoton excitation of the Blastochloris viridis photosynthetic reaction center, observing an ultrafast global conformational change that arises within picoseconds and precedes the propagation of heat through the protein. This provides direct structural evidence for a 'protein quake': the hypothesis that proteins rapidly dissipate energy through quake-like structural motions.

Biochemical Research Methods

FREQUENCY VIBRATIONAL-MODES

STRUCTURAL DYNAMICS

Författare

David Arnlund

Göteborgs universitet

Linda C Johansson

Göteborgs universitet

Cecilia Wickstrand

Göteborgs universitet

A. Barty

G. J. Williams

Erik Malmerberg

Göteborgs universitet

J Davidsson

D. Milathianaki

D. P. Deponte

R. L. Shoeman

D. J. Wang

D. James

Gergely Katona

Göteborgs universitet

Sebastian Westenhoff

Göteborgs universitet

T. A. White

A. Aquila

S. Bari

Peter Berntsen

Göteborgs universitet

M. Bogan

T. B. van Driel

R. B. Doak

K. S. Kjaer

M. Frank

R. Fromme

I. Grotjohann

R. Henning

M. S. Hunter

R. A. Kirian

I. Kosheleva

C. Kupitz

M. N. Liang

A. V. Martin

M. M. Nielsen

M. Messerschmidt

M. M. Seibert

Jennie Sjöhamn

Göteborgs universitet

F. Stellato

U. Weierstall

N. A. Zatsepin

J. C. H. Spence

P. Fromme

I. Schlichting

S. Boutet

G. Groenhof

H. N. Chapman

Richard Neutze

Göteborgs universitet

Nature Methods

1548-7091 (ISSN) 1548-7105 (eISSN)

Vol. 11 923-926

Ämneskategorier

Kemi

DOI

10.1038/nmeth.3067