Ethylene production in relation to nitrogen metabolism in Saccharomyces cerevisiae
Journal article, 2014

We have previously shown that ethylene production in Saccharomyces cerevisiae expressing the ethylene-forming enzyme (EFE) from Pseudomonas syringae is strongly influenced by variations in the mode of cultivation as well as the choice of nitrogen source. Here, we have studied the influence of nitrogen metabolism on the production of ethylene further. Using ammonium, glutamate, glutamate/arginine, and arginine as nitrogen sources, it was found that glutamate (with or without arginine) correlates with a high ethylene production, most likely linked to an observed increase in 2-oxoglutarate levels. Arginine as a sole nitrogen source caused a reduced ethylene production. A reduction of arginine levels, accomplished using an arginine auxotrophic ARG4-deletion strain in the presence of limiting amounts of arginine or through CAR1 overexpression, did however not correlate with an increased ethylene production. As expected, arginine was necessary for ethylene production as ethylene production in the ARG4-deletion strain ceased at the time when arginine was depleted. In conclusion, our data suggest that high levels of 2-oxoglutarate and a limited amount of arginine are required for successful ethylene production in yeast.

yeast

biotechnological

chemical

Author

Nina Johansson

Chalmers, Chemical and Biological Engineering, Life Sciences

Karl Persson

Chalmers, Chemical and Biological Engineering, Life Sciences

Paul Quehl

Systems and Synthetic Biology

Joakim Norbeck

Chalmers, Chemical and Biological Engineering, Life Sciences

Christer Larsson

Chalmers, Chemical and Biological Engineering, Life Sciences

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 14 7 1110-1118

Subject Categories

Bioinformatics and Systems Biology

DOI

10.1111/1567-1364.12208

More information

Latest update

11/23/2018