Ethylene production in relation to nitrogen metabolism in Saccharomyces cerevisiae
Artikel i vetenskaplig tidskrift, 2014

We have previously shown that ethylene production in Saccharomyces cerevisiae expressing the ethylene-forming enzyme (EFE) from Pseudomonas syringae is strongly influenced by variations in the mode of cultivation as well as the choice of nitrogen source. Here, we have studied the influence of nitrogen metabolism on the production of ethylene further. Using ammonium, glutamate, glutamate/arginine, and arginine as nitrogen sources, it was found that glutamate (with or without arginine) correlates with a high ethylene production, most likely linked to an observed increase in 2-oxoglutarate levels. Arginine as a sole nitrogen source caused a reduced ethylene production. A reduction of arginine levels, accomplished using an arginine auxotrophic ARG4-deletion strain in the presence of limiting amounts of arginine or through CAR1 overexpression, did however not correlate with an increased ethylene production. As expected, arginine was necessary for ethylene production as ethylene production in the ARG4-deletion strain ceased at the time when arginine was depleted. In conclusion, our data suggest that high levels of 2-oxoglutarate and a limited amount of arginine are required for successful ethylene production in yeast.

yeast

biotechnological

chemical

Författare

Nina Johansson

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Karl Persson

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Paul Quehl

Systembiologi

Joakim Norbeck

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Christer Larsson

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

FEMS Yeast Research

1567-1356 (ISSN) 1567-1364 (eISSN)

Vol. 14 7 1110-1118

Ämneskategorier

Bioinformatik och systembiologi

DOI

10.1111/1567-1364.12208

Mer information

Senast uppdaterat

2018-11-23