Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via beta-alanine
Journal article, 2015

Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharolnyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the beta-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of beta-alanine and its subsequent conversion into 3HP using a novel beta-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7 +/- 0.3 g L-1 with a 0.14 +/- 0.0 C-mol C-mol(-1) yield on glucose in 80 h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.


Saccharomyces cerevisiae

3-hydroxypropionic acid

Biosustainable acrylics

beta-alanine-pyruvate aminotransferase


I. Borodina

Technical University of Denmark (DTU)

K. R. Kildegaard

Technical University of Denmark (DTU)

N. B. Jensen

Technical University of Denmark (DTU)

T. H. Blicher

University of Copenhagen

J. Maury

Technical University of Denmark (DTU)

S. Sherstyk

Technical University of Denmark (DTU)

K. Schneider

Technical University of Denmark (DTU)

P. Lamosa

Instituto de Tecnologia Quimica e Biologica (ITQB)

M. J. Herrgard

Technical University of Denmark (DTU)

I. Rosenstand

Technical University of Denmark (DTU)

F. Oberg

Technical University of Denmark (DTU)

J. Forster

Technical University of Denmark (DTU)

Jens B Nielsen

Chalmers, Biology and Biological Engineering, Systems and Synthetic Biology

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 27 57-64

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

European Commission (EC) (EC/FP7/247013), 2010-01-01 -- 2014-12-31.

Subject Categories

Industrial Biotechnology

Areas of Advance


Life Science Engineering (2010-2018)



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5/8/2018 1