Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via beta-alanine
Artikel i vetenskaplig tidskrift, 2015

Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharolnyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the beta-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of beta-alanine and its subsequent conversion into 3HP using a novel beta-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7 +/- 0.3 g L-1 with a 0.14 +/- 0.0 C-mol C-mol(-1) yield on glucose in 80 h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.


Saccharomyces cerevisiae

3-hydroxypropionic acid

Biosustainable acrylics

beta-alanine-pyruvate aminotransferase


I. Borodina

Danmarks Tekniske Universitet (DTU)

K. R. Kildegaard

Danmarks Tekniske Universitet (DTU)

N. B. Jensen

Danmarks Tekniske Universitet (DTU)

T. H. Blicher

Köpenhamns universitet

J. Maury

Danmarks Tekniske Universitet (DTU)

S. Sherstyk

Danmarks Tekniske Universitet (DTU)

K. Schneider

Danmarks Tekniske Universitet (DTU)

P. Lamosa

Instituto de Tecnologia Quimica e Biologica (ITQB)

M. J. Herrgard

Danmarks Tekniske Universitet (DTU)

I. Rosenstand

Danmarks Tekniske Universitet (DTU)

F. Oberg

Danmarks Tekniske Universitet (DTU)

J. Forster

Danmarks Tekniske Universitet (DTU)

Jens B Nielsen

Chalmers, Biologi och bioteknik, Systembiologi

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 27 57-64

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

Europeiska kommissionen (EU) (EC/FP7/247013), 2010-01-01 -- 2014-12-31.


Industriell bioteknik



Livsvetenskaper och teknik (2010-2018)



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