Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

Forskningsprojekt, 2010 – 2014

Metabolic engineering is the development of new cell factories or improving existing ones, and it is the enabling science that allows for sustainable production of fuels and chemicals through biotechnology. With the development in genomics and functional genomics, it has become interesting to evaluate how advanced high-throughput experimental techniques (transcriptome, proteome, metabolome and fluxome) can be applied for improving the process of metabolic engineering. These techniques have mainly found applications in life sciences and studies of human health, and it is necessary to develop novel bioinformatics techniques and modelling concepts before they can provide physiological information that can be used to guide metabolic engineering strategies.In particular it is challenging how these techniques can be used to advance the use of mathematical modelling for description of the operation of complex metabolic networks. The availability of robust mathematical models will allow a wider use of mathematical models to drive metabolic engineering, in analogy with other fields of engineering where mathematical modelling is central in the design phase. In this project the advancement of novel concepts, models and technologies for enhancing metabolic engineering will be done in connection with the development of novel cell factories for high-level production of different classes of products. The chemicals considered will involve both commodity type chemicals like 3-hydroxypropionic acid and malic acid, that can be used for sustainable production of polymers, an industrial enzyme and pharmaceutical proteins like human insulin.

Deltagare

Finansiering

Relaterade styrkeområden och infrastruktur

Hållbar utveckling

Drivkrafter

Publikationer

Moderate Expression of SEC16 Increases Protein Secretion by Saccharomyces cerevisiae

Physiological and transcriptional characterization of Saccharomyces cerevisiae engineered for production of fatty acid ethyl esters

Long-chain Alkane Production by the Yeast Saccharomyces cerevisiae

Ach1 is involved in shuttling mitochondrial acetyl units for cytosolic C2 provision in Saccharomyces cerevisiae lacking pyruvate decarboxylase

Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain

Microbial acetyl-CoA metabolism and metabolic engineering

Metabolic engineering strategies for microbial synthesis of oleochemicals

Logical transformation of genome-scale metabolic models for gene level applications and analysis

Engineering the Oxygen Sensing Regulation Results in an Enhanced Recombinant Human Hemoglobin Production by Saccharomyces cerevisiae

Controllability analysis of transcriptional regulatory networks reveals circular control patterns among transcription factors

Improving heterologous protein secretion at aerobic conditions by activating hypoxia-induced genes in Saccharomyces cerevisiae

Production of natural products through metabolic engineering of Saccharomyces cerevisiae

Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine

Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via beta-alanine

Microfluidic screening and whole-genome sequencing identifies mutations associated with improved protein secretion by yeast

Thermotolerant Yeast Strains Adapted by Laboratory Evolution Show Trade-Off at Ancestral Temperatures and Preadaptation to Other Stresses

Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth

Management of the endoplasmic reticulum stress by activation of the heat shock response in yeast

Physiological characterization of the high malic acid-producing Aspergillus oryzae strain 2103a-68

BioMet Toolbox 2.0: genome-wide analysis of metabolism and omics data

Impact of protein uptake and degradation on recombinant protein secretion in yeast

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways

Improved Production of a Heterologous Amylase in Saccharomyces cerevisiae by Inverse Metabolic Engineering

Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae

Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae

Balanced globin protein expression and heme biosynthesis improve production of human hemoglobin in Saccharomyces cerevisiae

Improving Production of Malonyl Coenzyme A-Derived Metabolites by Abolishing Snf1-Dependent Regulation of Acc1

Mapping condition-dependent regulation of metabolism in yeast through genome-scale modeling

Genome-Scale Modeling of the Protein Secretory Machinery in Yeast

Investigation of Malic Acid Production in Aspergillus oryzae under Nitrogen Starvation Conditions

The role of biofuels in the future energy supply

The RAVEN Toolbox and Its Use for Generating a Genome-scale Metabolic Model for Penicillium chrysogenum

Advances in metabolic pathway and strain engineering paving the way for sustainable production of chemical building blocks

Correlation of cell growth and heterologous protein production by Saccharomyces cerevisiae

Anaerobic alpha-Amylase Production and Secretion with Fumarate as the Final Electron Acceptor in Saccharomyces cerevisiae

Production of biopharmaceutical proteins by yeast Advances through metabolic engineering

Toward systems metabolic engineering of Aspergillus and Pichia species for the production of chemicals and biofuels

Advanced biofuel production by the yeast Saccharomyces cerevisiae

yApoptosis: yeast apoptosis database

Heat shock response improves heterologous protein secretion in Saccharomyces cerevisiae

Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway

Adaptively evolved yeast mutants on galactose show trade-offs in carbon utilization on glucose

Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism

Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae

Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation

Dynamic (13) C-labelling experiments prove important differences in protein turnover rate between two Saccharomyces cerevisiae strains

Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress

Fifteen years of large scale metabolic modeling of yeast: Developments and impacts

Enhancing the copy number of episomal plasmids in Saccharomyces cerevisiae for improved protein production

Aspergilli: Systems biology and industrial applications

Scheffersomyces stipitis: a comparative systems biology study with the Crabtree positive yeast Saccharomyces cerevisiae

Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae

Recovery of phenotypes obtained by adaptive evolution through inverse metabolic engineering

A comprehensive comparison of RNA-Seq-based transcriptome analysis from reads to differential gene expression and cross-comparison with microarrays: a case study in Saccharomyces cerevisiae

Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae

Unravelling evolutionary strategies of yeast for improving galactose utilization through integrated systems level analysis

Molecular and process design for rotavirus-like particle production in Saccharomyces cerevisiae

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