Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism
Artikel i vetenskaplig tidskrift, 2013

Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of alpha-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.

sequence

Saccharomyces cerevisiae

saccharomyces-cerevisiae

Cell factory

synthetase

fermentation

expression

growth-rate

alpha-Santalene

transcription

precursor

pyruvate-decarboxylase

glucose

Acetyl-CoA

Författare

Yun Chen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

L. Daviet

Firmenich SA

M. Schalk

Firmenich SA

Verena Siewers

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Jens B Nielsen

Chalmers, Kemi- och bioteknik, Livsvetenskaper, Systembiologi

Metabolic Engineering

1096-7176 (ISSN) 1096-7184 (eISSN)

Vol. 15 48-54

Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)

Europeiska kommissionen (FP7), 2010-01-01 -- 2014-12-31.

Drivkrafter

Hållbar utveckling

Ämneskategorier

Biologiska vetenskaper

Styrkeområden

Energi

Livsvetenskaper och teknik

DOI

10.1016/j.ymben.2012.11.002