Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism
Journal article, 2013
Production of fuels and chemicals by industrial biotechnology requires efficient, safe and flexible cell factory platforms that can be used for production of a wide range of compounds. Here we developed a platform yeast cell factory for efficient provision of acetyl-CoA that serves as precursor metabolite for a wide range of industrially interesting products. We demonstrate that the platform cell factory can be used to improve the production of alpha-santalene, a plant sesquiterpene that can be used as a perfume by four-fold. This strain would be a useful tool to produce a wide range of acetyl-CoA-derived products.
Acetyl-CoA
Saccharomyces cerevisiae
saccharomyces-cerevisiae
pyruvate-decarboxylase
expression
Cell factory
growth-rate
precursor
synthetase
alpha-Santalene
sequence
glucose
transcription
fermentation
Author
Yun Chen
Chalmers, Chemical and Biological Engineering, Life Sciences
L. Daviet
Firmenich Inc.
M. Schalk
Firmenich Inc.
Verena Siewers
Chalmers, Chemical and Biological Engineering, Life Sciences
Jens B Nielsen
Chalmers, Chemical and Biological Engineering, Life Sciences
Metabolic Engineering
1096-7176 (ISSN) 1096-7184 (eISSN)
Vol. 15 1 48-54Industrial Systems Biology of Yeast and A. oryzae (INSYSBIO)
European Commission (EC) (EC/FP7/247013), 2010-01-01 -- 2014-12-31.
Driving Forces
Sustainable development
Subject Categories
Biological Sciences
Areas of Advance
Energy
Life Science Engineering (2010-2018)
DOI
10.1016/j.ymben.2012.11.002