Verena Siewers

2023

Functional genome annotation and transcriptome analysis of Pseudozyma hubeiensis BOT-O, an oleaginous yeast that utilizes glucose and xylose at equal rates

Fine-tuning of p-coumaric acid synthesis to increase (2S)-naringenin production in yeast

2023

Expanding the genetic toolbox of Rhodotorula toruloides by identification and validation of six novel promoters induced or repressed under nitrogen starvation

2023

Fine-tuning of p-coumaric acid synthesis to increase (2S)-naringenin production in yeast

Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae

2023

Multiplexed Guide RNA Expression Leads to Increased Mutation Frequency in Targeted Window Using a CRISPR-Guided Error-Prone DNA Polymerase in Saccharomyces cerevisiae

2023

Targeted In Vivo Mutagenesis in Yeast Using CRISPR/Cas9 and Hyperactive Cytidine and Adenine Deaminases

2023

A highly selective cell-based fluorescent biosensor for genistein detection

Saccharomyces cerevisiae as a Heterologous Host for Natural Products

2022

Genetically Encoded Whole Cell Biosensor for Drug Discovery of HIF-1 Interaction Inhibitors

2022

A p-Coumaroyl-CoA Biosensor for Dynamic Regulation of Naringenin Biosynthesis in Saccharomyces cerevisiae

2022

Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules

2022

Identification of a novel gene required for competitive growth at high temperature in the thermotolerant yeast Kluyveromyces marxianus

An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae

2022

Identification of acetic acid sensitive strains through biosensor-based screening of a Saccharomyces cerevisiae CRISPRi library

2022

Dataset for suppressors of amyloid-beta toxicity and their functions in recombinant protein production in yeast

A Hypersensitive Genetically Encoded Fluorescent Indicator (roGFP2-Prx1) Enables Continuous Measurement of Intracellular H2O2 during Cell Micro-cultivation

2022

Reconstruction of a catalogue of genome-scale metabolic models with enzymatic constraints using GECKO 2.0

2022

Controlling gene expression with deep generative design of regulatory DNA

2022

Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism

2022

CRISPR/Cas9-mediated point mutations improve alpha-amylase secretion in Saccharomyces cerevisiae

2021

A single chromosome strain of S. cerevisiae exhibits diminished ethanol metabolism and tolerance

Metabolic Engineering of Yeast

2021

The Yeast eIF2 Kinase Gcn2 Facilitates H2O2-Mediated Feedback Inhibition of Both Protein Synthesis and Endoplasmic Reticulum Oxidative Folding during Recombinant Protein Production

2021

Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

2021

Production of 10-methyl branched fatty acids in yeast

2021

Rational gRNA design based on transcription factor binding data

2021

Evaluating accessibility, usability and interoperability of genome-scale metabolic models for diverse yeasts species

GTR 2.0: GRNA-tRNA Array and Cas9-NG Based Genome Disruption and Single-Nucleotide Conversion in Saccharomyces cerevisiae

2021

Development of an Haa1-based biosensor for acetic acid sensing in Saccharomyces cerevisiae

2021

Transcriptomic response of Saccharomyces cerevisiae to octanoic acid production

2021

Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries

2020

Promiscuous phosphoketolase and metabolic rewiring enables novel non-oxidative glycolysis in yeast for high-yield production of acetyl-CoA derived products

2020

The transcription factor Leu3 shows differential binding behavior in response to changing leucine availability

2020

Stress-induced expression is enriched for evolutionarily young genes in diverse budding yeasts

Adaptive Laboratory Evolution of Ale and Lager Yeasts for Improved Brewing Efficiency and Beer Quality

2020

Different routes of protein folding contribute to improved protein production in saccharomyces cerevisiae

2020

Does co-expression of Yarrowia lipolytica genes encoding Yas1p, Yas2p and Yas3p make a potential alkane-responsive biosensor in Saccharomyces cerevisiae?

2020

Deep learning suggests that gene expression is encoded in all parts of a co-evolving interacting gene regulatory structure

Engineering carboxylic acid reductase for selective synthesis of medium-chain fatty alcohols in yeast

Multidimensional engineering of Saccharomyces cerevisiae for efficient synthesis of medium-chain fatty acids

Saccharomyces cerevisiae displays a stable transcription start site landscape in multiple conditions

2019

Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae

Strategies and challenges for metabolic rewiring

2019

Identification of genes involved in shea butter biosynthesis from Vitellaria paradoxa fruits through transcriptomics and functional heterologous expression

Expanding the Dynamic Range of a Transcription Factor-Based Biosensor in Saccharomyces cerevisiae

2019

Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals

FadR-Based Biosensor-Assisted Screening for Genes Enhancing Fatty Acyl-CoA Pools in Saccharomyces cerevisiae

2019

Integration of a multi-step heterologous pathway in Saccharomyces cerevisiae for the production of abscisic acid

2019

Model-Assisted Fine-Tuning of Central Carbon Metabolism in Yeast through dCas9-Based Regulation

2019

Increasing jojoba-like wax ester production in Saccharomyces cerevisiae by enhancing very long-chain, monounsaturated fatty acid synthesis

2019

Effects of overexpression of STB5 in Saccharomyces cerevisiae on fatty acid biosynthesis, physiology and transcriptome

2018

Metabolic engineering of Saccharomyces cerevisiae for overproduction of triacylglycerols

Heterologous transporter expression for improved fatty alcohol secretion in yeast

Dynamic engineering of 1-alkenes biosynthesis and secretion in yeast.

Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae

2018

Engineering microbial fatty acid metabolism for biofuels and biochemicals

2018

Redirection of lipid flux toward phospholipids in yeast increases fatty acid turnover and secretion

2018

Global rewiring of cellular metabolism renders Saccharomyces cerevisiae Crabtree negative

2018

Expression of cocoa genes in Saccharomyces cerevisiae improves cocoa butter production

Investigation of putative regulatory acetylation sites in Fas2p of Saccharomyces cerevisiae

2017

Cocoa butter-like lipid production ability of non-oleaginous and oleaginous yeasts under nitrogen-limited culture conditions

2017

Increasing cocoa butter-like lipid production of Saccharomyces cerevisiae by expression of selected cocoa genes

Evolutionary engineering reveals divergent paths when yeast is adapted to different acidic environments.

2017

Effects of acetoacetyl-CoA synthase expression on production of farnesene in Saccharomyces cerevisiae

2017

Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals

2017

Dynamic regulation of fatty acid pools for improved production of fatty alcohols in Saccharomyces cerevisiae

Affibody Scaffolds Improve Sesquiterpene Production in Saccharomyces cerevisiae.

Establishing very long-chain fatty alcohol and wax ester biosynthesis in Saccharomyces cerevisiae

Metabolic Engineering Strategies to Convert Carbohydrates to Aviation Range Hydrocarbons

2016

Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae

Harnessing yeast peroxisomes for biosynthesis of fatty-acid-derived biofuels and chemicals with relieved side-pathway competition

Production of farnesene and santalene by Saccharomyces cerevisiae using fed-batch cultivations with RQ-controlled feed

Improved production of fatty acids by Saccharomyces cerevisiae through screening a cDNA library from the oleaginous yeast Yarrowia lipolytica

2016

Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories

Flux Control at the Malonyl-CoA Node through Hierarchical Dynamic Pathway Regulation in Saccharomyces cerevisiae

Physiological and transcriptional characterization of Saccharomyces cerevisiae engineered for production of fatty acid ethyl esters

Biosynthesis of very long-chain fatty alcohols and wax esters in metabolically engineered strains of Saccharomyces cerevisiae

2015

Editorial: Yeast synthetic biology: new tools to unlock cellular function

Advances in yeast genome engineering

2015

Molecular Mechanism of Flocculation Self-Recognition in Yeast and Its Role in Mating and Survival

Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth

2015

Production of beta-ionone by combined expression of carotenogenic and plant CCD1 genes in Saccharomyces cerevisiae

Microbial acetyl-CoA metabolism and metabolic engineering

2015

Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine

2015

RNA-seq analysis of Pichia anomala reveals important mechanisms required for survival at low pH

Enabling Technologies to Advance Microbial Isoprenoid Production

Ach1 is involved in shuttling mitochondrial acetyl units for cytosolic C2 provision in Saccharomyces cerevisiae lacking pyruvate decarboxylase

2015

Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain

Long-chain Alkane Production by the Yeast Saccharomyces cerevisiae

Metabolic pathway engineering for fatty acid ethyl ester production in Saccharomyces cerevisiae using stable chromosomal integration

2014

Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol degradation pathway and expression of the heterologous phosphoketolase pathway

2014

Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid ethyl esters, an advanced biofuel, by eliminating non-essential fatty acid utilization pathways

Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae

2014

Improving Production of Malonyl Coenzyme A-Derived Metabolites by Abolishing Snf1-Dependent Regulation of Acc1

Engineering of Chromosomal Wax Ester Synthase Integrated Saccharomyces Cerevisiae Mutants for Improved Biosynthesis of Fatty Acid Ethyl Esters

2014

Enhanced ethanol production and reduced glycerol formation in fps1∆ mutants of Saccharomyces cerevisiae engineered for improved redox balancing

A universal fixation method based on quaternary ammonium salts (RNAlater) for omics-technologies: Saccharomyces cerevisiae as a case study

Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism

Improving biobutanol production in engineered Saccharomyces cerevisiae by manipulation of acetyl-CoA metabolism

From flavors and pharmaceuticals to advanced biofuels: Production of isoprenoids in Saccharomyces cerevisiae

Advanced biofuel production by the yeast Saccharomyces cerevisiae

Improved polyhydroxybutyrate production by Saccharomyces cerevisiae through the use of the phosphoketolase pathway

Systems biology of yeast: enabling technology for development of cell factories for production of advanced biofuels

2012

Profiling of Cytosolic and Peroxisomal Acetyl-CoA Metabolism in Saccharomyces cerevisiae

2012

Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production

dynamic control of gene expression in saccharomyces cerevisiae engineered for the production of plant sesquiterpene alpha-santalene in a fed-batch mode

A systems-level approach for metabolic engineering of yeast cell factories

2012

Combined metabolic engineering of precursor and co-factor supply to increase α-santalene production by Saccharomyces cerevisiae

Physiological characterization of recombinant Saccharomyces cerevisiae expressing the Aspergillus nidulans phosphoketolase pathway: validation of activity through (13)C-based metabolic flux analysis.

2012

Reconstruction and Evaluation of the Synthetic Bacterial MEP Pathway in Saccharomyces cerevisiae

2012

Engineering of acetyl-CoA metabolism for the improved production of polyhydroxybutyrate in Saccharomyces cerevisiae

Enhancing the copy number of episomal plasmids in Saccharomyces cerevisiae for improved protein production

The Influence of Microgravity on Invasive Growth in Saccharomyces cerevisiae

2011

Prospects for microbial biodiesel production

Opportunities for yeast metabolic engineering: Lessons from synthetic biology

Characterization of different promoters for designing a new expression vector in Saccharomyces cerevisiae

Implementation of communication-mediating domains for non-ribosomal peptide production in Saccharomyces cerevisae

Phosphoglycerate mutase knock-out mutant Saccharomyces cerevisiae: Physiological investigation and transcriptome analysis

Characterization of chromosomal integration sites for heterologous gene expression in Saccharomyces cerevisiae

Heterologous production of non-ribosomal peptide LLD-ACV in Saccharomyces cerevisiae